Summary
Spontaneous activity of the isolated taenia coli of the guinea-pig was measured with various techniques: extracellular recording of electrical activity with Pt-electrodes and with the sucrose gap technique; intracellular measurement of membrane potential with glass microelectrodes; recording of mechanical activity under isometric, isotonic and auxotonic conditions. Normal spontaneous activity is always characterized by slow fluctuations with a period duration of 1–3 min. This “minute-rhythm” is also present in the taenia coli in situ, as measurements with implanted Pt-electrodes have shown. In strongly stretched and very short preparations (less than 5 mm) the minute-rhythm usually disappears. This may be the reason why the minute-rhythm was not observed in most of the recent investigations of taenia coli. Many influences on the spontaneous activity of taenia coli must be reexamined with consideration of the minute-rhythm. The classification of the rhythmic phenomena in smooth muscle is discussed.
Zusammenfassung
Die Spontanaktivität der isolierten Taenia coli des Meerschweinchens wurde mit verschiedenen Methoden registriert: extracelluläre Messung der elektrischen Aktivität mit Pt-Elektroden sowie mit der Saccharosetrennwand-Technik; intracelluläre Potentialmessung mit Glas-Mikroelektroden; Messung der mechanischen Aktivität unter isometrischen, isotonischen und auxotonischen Bedingungen. Die normale Spontanaktivität ist stets durch langsame Fluktuationen mit einer Periodendauer von 1–3 min gekennzeichnet. Auch in situ zeigt die Taenia coli diese “Minuten-Rhythmik”, wie Messungen mit implantierten Elektroden ergeben haben. Bei stark gedehnten und sehr kurzen Präparaten (unter 5 mm) erlischt die Minuten-Rhythmik in der Regel und gibt einer kontinuierlichen Dauerentladung von Spikes Raum. Dies ist offenbar der Hauptgrund dafür, daß in den meisten neueren Untersuchungen an der Taenia coli die Minuten-Rhythmik nicht beobachtet worden ist. Viele Einflüsse auf die Spontanaktivität der Taenie müssen unter Einbeziehung der Minuten-Rhythmik neu überprüft werden. Die Gliederung der verschiedenen rhythmischen Erscheinungen an der Taenie wird diskutiert.
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Golenhofen, K., Loh, D.v. Elektrophysiologische Untersuchungen zur normalen Spontanaktivität der isolierten Taenia coli des Meerschweinchens. Pflugers Arch. 314, 312–328 (1970). https://doi.org/10.1007/BF00592289
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DOI: https://doi.org/10.1007/BF00592289
Key-Words
- Electrophysiology of Smooth Muscle
- Spontaneous Activity of Intestinal Smooth Muscle
- Minute-Rhythm
- Intracellular Recording of Membrane Potential in Smooth Muscle