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Plasma insulin and carbohydrate metabolism after sucrose ingestion during rest and prolonged aerobic exericise

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RQ, plasma insulin, blood glucose, lactate and pyruvate were measured in six fit, normal subjects during a series of exercise and rest experiments with and without sucrose ingestion. Subjects exercised on a bicycle ergometer for 50 min of every hour for 6 h at about 47% of the group's average maximal aerobic capacity. In the resting experiments, the subjects sat for 6 h in an armchair. A solution containing 100 g of sucrose was ingested at the beginning of the fourth hour during the sucrose experiments. Ingestion of sucrose caused a significant increase in RQ, plasma insulin, blood glucose, lactate and pyruvate in both exercise and rest experiments. Insulin, lactate and pyruvate concentrations rose higher during rest after sucrose ingestion than during exercise. The time courses of the changes in RQ, insulin, glucose, lactate and pyruvate after sucrose ingestion, suggest that glucose entering the cell during rest is immediately oxidized, while during work there is some delay in the oxidation of glucose.

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References

  1. Bachmann, G., Haldi, J.: A comparative study of the respiratory quotient following the ingestion of glucose and of fructose as affected by the lactic acid and carbon dioxide changes in the blood. J. Nutr.13, 157 (1937)

    Google Scholar 

  2. Barker, S. B., Summerson, W. H.: The calorimetric determination of lactic acid in biological material. J. biol. Chem.138, 535 (1941)

    Google Scholar 

  3. Benadé, A. J. S., Jansen, C. R., Rogers, G. G., Wyndham, C. H., Strydom, N. B.: The significance of an increased RQ after sucrose ingestion during prolonged aerobic exercise. Pflügers Arch.342, 199–206 (1973)

    Google Scholar 

  4. Benadé, A. J. S., Strydom, N. B., van der Walt, W. H.: A comparison of physical and chemical methods for the determination of respiratory quotient. Int. Z. angew. Physiol.28, 193 (1970)

    Google Scholar 

  5. Benadé, A. H. S., Wyndham, C. H., Strydom, N. B., Rogers, G. G.: The physiological effects of a mid-shift feed of sucrose. S. Afr. med. J.45, 711 (1971)

    Google Scholar 

  6. Davies, C. T. M., Knibbs, A. V., Musgrove, J.: The rate of lactic acid removal in relation to different baselines of recovery exercise. Int. Z. angew. Physiol.28, 155 (1970)

    Google Scholar 

  7. Edwards, H. T., Bensley, E. H., Dill, D. B., Carpenter, T. M.: Human respiratory quotients in relation to alveolar carbon dioxide and blood lactic acid after ingestion of glucose, fructose or galactose. J. Nutr.27, 241 (1944)

    Google Scholar 

  8. Fordtran, J. S., Saltin, B.: Gastric emptying and intestinal absorption during prolonged severe exercise. J. appl. Physiol.23, 331 (1967)

    Google Scholar 

  9. Friedemann, T. E., Haugen, G. E.: Pyruvic acid II. The determination of keto acids in blood and urine. J. biol. Chem.147, 415 (1943)

    Google Scholar 

  10. Garland, P. B., Shepherd, D., Yates, D. W.: Steady-state concentrations of coenzyme A, acetyl-coenzyme A and long-chain fatty acyl-coenzyme A in rat liver mitochondria oxidizing palmitate. Biochem. J.97, 587 (1965)

    Google Scholar 

  11. Häggendal, J., Hartley, L. H., Saltin, B.: Arterial noradrenaline concentration during exercise in relation to the relative work levels. Scand. J. clin. Lab. Invest.26, 337 (1970)

    Google Scholar 

  12. Hales, C. N., Randle, P. J.: Immunoassay of insulin with insulin antibody precipitate. Biochem. J.88, 137 (1963)

    Google Scholar 

  13. Hermansen, L., Hultman, E., Saltin, B.: Muscle glycogen during prolonged severe exercise. Acta physiol. scand.71, 129 (1967)

    Google Scholar 

  14. Hermansen, L., Stensvold, I.: Production and removal of lactate during exercise in man. Acta physiol. scand.86, 191 (1972)

    Google Scholar 

  15. Huckabee, W. E.: Control of concentration gradients of pyruvate and lactate across cell membranes in blood. J. appl. Physiol.9, 163 (1956)

    Google Scholar 

  16. Hullin, R. P., Noble, R. L.: The determination of lactic acid in microgram quantities. Biochem. J.55, 289 (1953)

    Google Scholar 

  17. Hultman, E.: Studies on muscle metabolism of glycogen and active phosphate in man with special reference to exercise and diet. Scand. J. clin. Lab. Invest.19, 1 (1967)

    Google Scholar 

  18. Hultman, E., Bergström, J., Roch-Norlund, A. E.: Glycogen storage in human skeletal muscle. In: Muscle metabolism during exercise, pp. 273. New York: Plenum Press 1971

    Google Scholar 

  19. Hyvarinen, A., Nikkilä, E. A.: Specific determination of blood glucose with o-toluidine. Clin. chim. Acta7, 140 (1962)

    Google Scholar 

  20. Karlsson, J., Diamant, B., Saltin, B.: Muscle metabolites during submaximal and maximal exercise in man. Scand. J. clin. Lab. Invest.26, 385 (1970)

    Google Scholar 

  21. Kraegen, E. W., Chisholm, D. J., Young, J. D., Lazarus, L.: The gastrointestinal stimulus to insulin release. J. clin. Invest.48, 1453 (1969)

    Google Scholar 

  22. Madison, L. L., Combes, B., Unger, R. H., Kaplan, N.: The relationship between the mechanism of action of the sulfonylureas and the secretion of insulin into the portal circulation. Ann. N. Y. Acad. Sci.74, 548 (1959)

    Google Scholar 

  23. Mortimore, G. E., Tietze, F.: Studies on the mechanism of capture and degradation of insulin-I-131 by the cyclically perfused rat liver. Ann. N. Y. Acad. Sci.82, 329 (1959)

    Google Scholar 

  24. Nikkilä, E. A., Taskinen, M. R., Miettinen, T. A., Pelkonen, R., Poppius, H.: Effect of muscular exercise on insulin secretion. Diabetes17, 209 (1968)

    Google Scholar 

  25. Peterson, D. T., Reaven, G. M.: Evidence that glucose load is an important determinant of plasma insulin response in normal subjects. Diabetes20, 729 (1972)

    Google Scholar 

  26. Porte, D., Graber, A. L., Kuzuya, T., Williams, R. H.: The effect of epinephrine on immuno-reactive insulin levels in man. J. clin. Invest.45, 228 (1966)

    Google Scholar 

  27. Pruett, E. D. R., Oseid, S.: Effect of exercise on glucose and insulin response to glucose infusion. Scand. J. clin. Lab. Invest.26, 277 (1970)

    Google Scholar 

  28. Randle, P. J., Garland, P. B., Hales, C. N., Newsholme, E. A., Denton, R. M., Pogson, C. I.: Interactions of metabolism and physiological role of insulin. Recent. Progr. Hormone Res.22, 1 (1966)

    Google Scholar 

  29. Reinheimer, W., Davidson, P. C., Albrink, M. J.: Effect of moderate exercise on plasma glucose, insulin, and free fatty acids during oral glucose tolerance tests. J. Lab. clin. Med.71, 429 (1968)

    Google Scholar 

  30. Rowell, L. B., Blackman, J. R., Bruce, R. A.: Indocyanine green clearance and estimated hepatic blood flow during mild to maximal exercise in upright man. J. clin. Invest.43, 1677 (1964)

    Google Scholar 

  31. Saltzman, H. A., Salzano, J. V.: Effects of carbohydrate metabolism upon respiratory gas exchange in normal men. J. appl. Physiol.30, 228 (1971)

    Google Scholar 

  32. Seltzer, H. S., Allen, E. W., Herron, A. L., Jr., Brennan, M. T.: Insulin secretion in response to glycemic stimulus: Relation of delayed initial release to carbohydrate intolerance in mild diabetis mellitus. J. clin. Invest.46, 323 (1967)

    Google Scholar 

  33. Seymore, G. E. J., de Bruin, E. J. P.: Normal blood glucose tolerance curves and concomitant plasma insulin levels in the white South African. S. Afr. med. J.44, 1075 (1970)

    Google Scholar 

  34. Strydom, N. B., Cooke, H. M., Miller, H. D., Winer, P.: Errors in respiratory gas analysis. A comparison of the Haldane and Pauling gas analysers. Int. Z. angew. Physiol.21, 13 (1965)

    Google Scholar 

  35. Walker, D. G.: Animal hexokinase. In: Essays in Biochemistry, Vol. 2, p. 33. London 1966

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Benadé, A.J.S., Wyndham, C.H., Jansen, C.R. et al. Plasma insulin and carbohydrate metabolism after sucrose ingestion during rest and prolonged aerobic exericise. Pflugers Arch. 342, 207–218 (1973). https://doi.org/10.1007/BF00591369

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