Skip to main content
SpringerLink
Log in

In vitro binding of triiodothyronine to rat liver mitochondria

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Abstract

Saturable high affinity T3 binding sites were detected in a mitochondrial fraction enriched in internal membranes and partly solubilized by Triton X-100. Specific T3 binding to the solubilized sites, only detected at low T3 concentrations, was optimal at pH 8.0 and not dependent upon the presence of divalent cations or reducing agents; it was destroyed by heat and proteolytic enzymes. The solubilized T3 binding sites were distributed, after Sephadex G-200 gel filtration, between two peaks of similar affinity for T3 (Ka≃5×1010 l/mol) and similar binding characteristics. T3 was bound with a high stereospecificity, while some analogues of biological importance (l-T4; 3,5,3′-triiodothyroacetic acid: 3,3′-diiodo-l-thyronine) competed withl-T3 in the same range of low concentrations. This suggests that the high affinity mitochondrial T3 binding sites could be of biological relevance in the mitochondrial metabolism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Barletta A, Liverini G, Goglia F, DiMeo S, De Leo T (1980) Thyroid state and mitochondrial population during maturation and ageing. J Endocrinol Invest 3:293

    Google Scholar 

  2. Barsano CP, DeGroot LJ, Getz GS (1977) The effect of thyroid hormone on in vitro rat liver mitochondrial RNA synthesis. Endocrinology 100:52–60

    Google Scholar 

  3. Berg P, Boman D (1973) Distribution of lysosomal enzymes between parenchymal and Kupffer cells of rat liver. Biochim Biophys Acta 321:585–596

    Google Scholar 

  4. Bernal J, Refetoff S (1977) The action of thyroid hormone. Clin Endocrinol 6:227–249

    Google Scholar 

  5. Bouhnik J, Clot JP, Baudry M, Michel R (1979) Early effects of thyroidectomy and triiodothyronine administration on rat liver mitochondria. Mol Cell Endocrinol 15:1–12

    Google Scholar 

  6. Caplan AI, Greenawalt JW (1966) Biochemical and ultrastructural properties of osmotically lysed rat liver mitochondria. J Cell Biol 31:455–472

    Google Scholar 

  7. DeGroot LJ, Torresani J (1975) Triiodothyronine binding to isolated liver cell nuclei. Endocrinology 96:357–369

    Google Scholar 

  8. De Leo T, Di Meo S, Barletta A, Martino G, Goglia F (1976) Modification of nuclei acid levels per mitochondrion induced by thyroidectomy or triiodothyronine administration. Pflügers Arch 366:73–77

    Google Scholar 

  9. Docter D, Visser TJ, Stinis JT, Van den Hout-Goemaat NL, Hennemann, G (1976) Binding ofl-triiodothyronine to isolated rat liver and kidney nuclei under various circumstances. Acta Endocrinol 81:82–95

    Google Scholar 

  10. Gadaleta MN, Barletta A, Caldarazzo M, De Leo T, Saccone C (1972) Triiodothyronine action on RNA synthesis in rat liver mitochondria. Eur J Biochem 30:376–381

    Google Scholar 

  11. Gadaleta MN, Di Reda N, Bove G, Saccone C (1975) Effects of triiodothyronine on rat liver mitochondrial transcription process. Eur J Biochem 51:495–501

    Google Scholar 

  12. Gharbi J, Torresani J (1979) High affinity thyroxine binding to purified rat liver plasma membranes. Biochem Biophys Res Commun 88:170–177

    Google Scholar 

  13. Goglia F, Liverini G, Barletta A, Di Meo S, Torresani J, De Leo T (1978) Recettori mitocondriali epatici per la T3. Congresso delle Società italiana di Biologia Sperimentale (SIBS), di Fisiologia (SIF), di Nutrizione Umana (SINU), Ferrara, Italia. Abstract no. 38, Sett

  14. Greif RL (1977) Mitochondrial binding sites for triiodothyronine, 53rd Meeting of the American Thyroid Association, Cleveland, Ohio (Abstract T19)

  15. Greif RL, Sloane D (1978) Mitochondrial bindings sites for triiodothyronine. Endocrinology 103:1899–1902

    Google Scholar 

  16. Hoch FL (1968) Thyroid hormone action on mitochondria. I. Effect of inhibitors of respiration. Arch Biochem Biophys 124:238–247

    Google Scholar 

  17. Ji TH (1973) Interference by detergents, chelating agents and buffers with the Lowry protein determination. Anal Biochem 52:517–521

    Google Scholar 

  18. Koerner D, Schwartz HL, Surks MI, Oppenheimer JH, Jorgensen EC (1975) Binding of selected iodothyronine analogues to receptor sites in isolated rat hepatic nuclei. J Biol Chem 250:6417–6423

    Google Scholar 

  19. Lowry OH, Rosenbrough NJ, Farr AL Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  20. Oppenheimer JH, Koerner D, Schwartz HL, Surks MI (1972) Specific nuclear triiodothyronine binding sites in rat liver and kidney. J Clin Endocrinol Metab 35:330–333

    Google Scholar 

  21. Oppenheimer JH, Dillmann WH, Schwartz HL, Towle HC (1979) Nuclear receptors and thyroid hormone action: a progress report. Fed Proc 38:2154–2161

    Google Scholar 

  22. Parsons DF, Williams GR, Chance B (1966) Characteristics of isolated and purified preparations of the outer and inner membranes of mitochondria. Ann NY Acad Sci 137:643–666

    Google Scholar 

  23. Pliam NB, Goldfine ID (1977) High affinity thyroid hormone binding sites on purified rat liver plasma membranes. Biochem Biophys Res Commun 79:166–172

    Google Scholar 

  24. Portnay GI, McClendon FD, Bush JE, Braverman LE, Babior BM (1973) The effect of physiological doses of thyroxine on carrier mediated ADP uptake by liver mitochondria from thyroidectomized rats. Biochem Biophys Res Commun 55:17–21

    Google Scholar 

  25. Scatchard G (1949) The attraction of proteins for small molecules and ions. Ann NY Acad Sci 51:660–672

    Google Scholar 

  26. Sterling K, Milch PO (1975) Thyroid hormone binding by a component of mitochondrial membrane. Proc Nalt Acad Sci USA 72:3225–3229

    Google Scholar 

  27. Sterling K, Milch PO, Brenner MA, Lazarus JH (1977) Thyroid hormone action: the mitochondrial pathway. Science 197:996–999

    Google Scholar 

  28. Sterling K, Lazarus JH, Milch PO, Sakurada T, Brenner MA (1978) Mitochondrial thyroid hormone receptor: localization and physiological significance. Science 201:1126–1129

    Google Scholar 

  29. Tata JR (1975) How specific are nuclear “receptors” for thyroid hormones? Nature (London) 257:18–23

    Google Scholar 

  30. Torresani J, DeGroot LJ (1975) Triiodothyronine binding to liver nuclear solubilized proteins in vitro. Endocrinology 96:1201–1209

    Google Scholar 

  31. Torresani J, Anselmet A (1978) Partial purification and characterization of nuclear triiodothyronine binding proteins. Biochem Biophys Res Commun 81:147–153

    Google Scholar 

  32. Torresani J, Anselmet A, Wahl R (1978) Properties of solubilized nuclear triiodothyronine binding proteins. Mol Cell Endocrinol 9:321–333

    Google Scholar 

  33. Yoshizato K, Kistler A, Frieden E (1975) Binding of thyroid hormones by nuclei of cells from bullfrog tadpole tail fins. Endocrinology 97:1030–1035

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Fisiologia Generale dell'Università di Napoli, Via Mezzocannone, 8, I-80134, Napoli, Italy

    F. Goglia, P. Bugli, A. Barletta & G. Liverini

  2. Faculté de Médecine, Laboratoire de Biochimie Médicale et U 38 INSERM, Marseille, France

    J. Torresani

Authors
  1. F. Goglia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Torresani
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Bugli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Barletta
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. Liverini
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Goglia, F., Torresani, J., Bugli, P. et al. In vitro binding of triiodothyronine to rat liver mitochondria. Pflugers Arch. 390, 120–124 (1981). https://doi.org/10.1007/BF00590193

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00590193

Key words

Search

Navigation