Abstract
The renal actions of the optically active isomers of the new diuretic drug ozolinone were studied by clearance, flowmeter and micropuncture techniques in rats. The levorotatory, but not the dextrorotatory isomer of ozolinone increased urine flow, urinary sodium and chloride excretion and enhanced sodium and chloride concentrations in early distal tubular fluid as checked by in situ microperfusion of Henle's loops. This indicates stereospecifity of the drug's diuretic action. However, both isomers of ozolinone equally inhibited maximal tubular secretion of paraaminohippurate and increased renal blood flow as measured by an electromagnetic flowmeter. Thus, the different renal target structures of ozolinone differ markedly with respect to stereoselectivity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Burg, M., Stoner, L., Cardinal, J., Green, N.: Furosemide effect on isolated perfused tubules. Am. J. Physiol.225, 119–124 (1973)
Burg, M., Green, N.: Effect of ethacrynic acid on the thick ascending limb of Henle's loop. Kidney Int.4, 301–308 (1973)
Burg, M.: Tubular chloride transport and the mode of action of some diuretics. Kidney Int.9, 189–197 (1976)
Burg, M.: Mechanisms of action of diuretic drugs. In: The Kidney, Brenner and Rector (eds.), p. 737. Philadelphia: Saunders 1976
Epstein, F. H., Silva, P., Stoff, J.: Energetic aspects of chloride transport powered by Na-K-ATPase in the rectal gland. Proceedings VIIth International Congress of Nephrology, Montreal 1978, pp. 155–159
Friedman, P. A., Roch-Ramel, F.: Hemodynamic and natriuretic effects of bumetanide and furosemide in the cat. J. Pharm. Exp. Ther.203, 82–91 (1977)
Führ, J., Kaczmarczyk, J., Krüttgen, C. D.: Eine einfache Methode zur Inulinbestimmung für Nieren-Clearance-Untersuchung bei Stoffwechselgesunden und Diabetikern. Klin. Wochenschr.33, 729–730 (1955)
Goldberg, M.: The renal physiology of diruretics. In: Handbook or physiology, Chapter 28, pp. 1003–1031. Baltimore, Maryland: The Williams and Wilkins Company 1973
Greven, J., heidenreich, O.: Effect of etozoline on whole kidney function and fluid and electrolyte reabsorption in rat proximal convoluted tubules and loops of Henle. Arzneim.-Forsch. (Drug. Res.)27, 1755–1757 (1977)
Greven, J., Heidenreich, O.: Effects of ozolinone, a diuretic active metabolite of etozoline, on renal function. I. Clearance studies in dogs. Naunyn-Schmiedeberg's Arch. Pharmacol.304, 283–287 (1978)
Greven, J., Klein, H., Heidenreich, O.: Effects of ozolinone, a diuretic active metabolite of etozoline, on renal function. II. Localization of tubular site of diuretic action by micropuncture in the rat. Naunyn-Schmiedeberg's Arch. Pharmacol.304, 289–296 (1978)
Heidenreich, O., Baumeister, L.: Über die additive Wirkung von chemisch und wirkungsmäßig unterschiedlichen Diuretika. Klin. Wochenschr.42, 1236–1240 (1964)
Heidenreich, O., Fülgraff, G., Baumeister, L., Schmiz, K.: Lokalisierung der Wirkungsorte des Diuretikums Etozolin im Nephron des Hundes und Mitteilung einer rechnerischen Auswertungsweise von stop-flow-Versuchen. Arch. Exp. Path. Pharmakol.249, 432–445 (1964)
Heidenreich, O., Gharemani, G., Keller, P., Kook, Y., Schmiz, K.: Die Wirkungen von 2-Carbäthoxymethylen-3-methyl-5-N-piperidinothiazolidin-4 auf die Nierenfunktion von Ratten und Hunden. Arzneim.-Forsch.14, 1242–1248 (1964)
Herrmann, M.: unpublished results
Hook, J. B., Williamson, H. E.: Influence of probenecid and alterations in acid-base balance on the saluretic activity of furosemide. J. Pharmacol. Exp. Ther.149, 404–408 (1965)
Jacobsen, H. R., Kokko, J. P.: Diuretics: Sites and mechanisms of action. Ann. Rev. Pharmacol. Toxicol.16, 201–215 (1976)
Ramsay, J. A., Brown, R. H. J., Croghan, P. C.: Electrometric titration of chloride in small volumes. J. Exp. Biol.32, 822–824 (1955)
Satzinger, G., Herrmann, W.: unpublished results
Satzinger, G., Herrmann, M., Vollmer, K.-O., Merzweiler, A., Gohmar, H., Heidenreich, O., Greven, J.: Etozoline: a novel diuretic. In: „Diuretic agents”, E. J. Cragoe (ed.), pp. 155–189. Washington, D. C.: American Chemical Society 1978
Seely, J. F., Dirks, J. H.: Site of action of diuretic drugs. Kidney Int.11, 1–8 (1977)
Schmidt, U., Dubach, U. C.: The behavior of Na+, K+ activated adenosine triphosphatase on various structures of the rat nephron after furosemide application. Nephron7, 447–458 (1970)
Smith, H. W.: Principles of renal physiology. New York: Oxford University Press 1956
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Greven, J., Defrain, W., Glaser, K. et al. Studies with the optically active isomers of the new diuretic drug ozolinone. Pflugers Arch. 384, 57–60 (1980). https://doi.org/10.1007/BF00589514
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00589514