Summary
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1.
22Sodium efflux in cardiac Purkyně fibres was found to be extremely rapid. The efflux curve obtained at 37° C was far from being a single exponential process.
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The volumes of distribution of inulin, sucrose and mannitol were measured. Mannitol equilibrated very quickly with a space of 697 ml/kg wet wt. Sucrose equilibrated somewhat slower and inulin space was still increasing after 2 h incubation.
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By lowering the efflux temperature it was possible to dissociate between intra- and extracellular sodium. The fast component of the Na efflux corresponded to the mannitol space. Taking the slow Na component, at low temperature, as representing the intracellular Na exchange one can calculate an intracellular Na concentration of 25 mM/l.
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4.
The rate of exchange of intracellular Na at 37° C, as estimated in two different ways, amounts to 0.14 min−1. The transmembrane Na efflux was calculated to be 15 pM cm−2sec−1.
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Bosteels, S., Carmeliet, E. Estimation of intracellular Na concentration and transmembrane Na flux in cardiac Purkyně fibres. Pflugers Arch. 336, 35–47 (1972). https://doi.org/10.1007/BF00589140
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DOI: https://doi.org/10.1007/BF00589140