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Paternal leakage of mitochondrial DNA inPinus

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Summary

We studied mitochondrial DNA restriction fragment length polymorphism in 11 parents and 125 seedlings of 23 controlled matings within and between jack pine (Pinus banksiana Lamb.) and lodgepole pine (P. contorta Dougl.). A potential mitochondrial distinction between these two conifers was evident in the parental samples. Only maternal mitochondrial restriction fragments were observed in a majority of the seedlings, which is consistent with results from angiosperms and other members of the genusPinus L. However, we detected exclusively paternal mitochondrial DNA in six of the seedlings. These unusual seedlings were not attributable to heteroplasmy or contamination of the experimental material, indicating that mitochondrial inheritance was not strictly maternal. Paternal mitochondrial leakage inPinus may permit novel insights into the transmission genetics and evolution of organellar polymorphisms.

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References

  • Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids ofNicotiana tabacum by protoplast fusion. Nature 281:401–403

    Google Scholar 

  • Birky CW Jr, Fuerst P, Maruyama T (1989) Organelle gene diversity under migration, mutation, and drift: equilibrium expectations, approach to equilibrium, effects of heteroplasmic cells, and comparison to nuclear genes. Genetics 121:613–627

    Google Scholar 

  • Bruns D, Owens JN (1989) Mechanisms of cytoplasmic inheritance in western white pine. In: Bailey GW (ed) Proc 47th Annu Meeting Electron Microscopy Soc America. San Francisco Press, San Francisco, pp 766–767

    Google Scholar 

  • Chesnoy L (1987) L'origine des organites du cytoplasme embryonnaire chez les gymnospermes. Bull Soc Bot Fr, Actual Bot 134:51–56

    Google Scholar 

  • Chiu WL, Stubbe W, Sears BB (1988) Plastid inheritance inOenothera: organelle genome modifies the extent of biparental plastid transmission. Curr Genet 13:181–189

    Google Scholar 

  • Correns C (1909) Vererbungsversuche mit blass (gelb) grünen und buntblättrigen Sippen beiMirabilis jalapa, Urtica pilulifera undLunaria annua. Z Vererbungsl 1:291–329

    Google Scholar 

  • Critchfield WB (1957) Geographic variation inPinus contorta. Maria Cabot Moors Foundation, Cambridge

    Google Scholar 

  • Dawson AJ, Jones VP, Leaver CJ (1986) Strategies for the identification and analysis of higher plant mitochondrial genes. Methods Enzymol 118:470–485

    Google Scholar 

  • Fox TD, Leaver CJ (1981) TheZea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons. Cell 26:315–323

    Google Scholar 

  • Govindaraju DR, Wagner DB, Smith GP, Dancik BP (1988) Chloroplast DNA variation within individual trees of aPinus banksiana — Pinus contorta sympatric region. Can J For Res 18:1347–1350

    Google Scholar 

  • Harrison RG, Doyle JJ (1990) Redwoods break the rules. Nature 344:295–296

    Google Scholar 

  • Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325

    Google Scholar 

  • Neale DB, Sederoff RR (1988) Inheritance and evolution of conifer organelle genomes. In: Hanover JW, Keathley DE (eds) Genetic manipulation of woody plants. Plenum Press, New York, pp 251–264

    Google Scholar 

  • Neale DB, Sederoff RR (1989) Paternal inheritance of chloroplast DNA and maternal inheritance of mitochondrial DNA in loblolly pine. Theor Appl Genet 77:212–216

    Google Scholar 

  • Neale DB, Wheeler NC, Allard RW (1986) Paternal inheritance of chloroplast DNA in Douglas-fir. Can J For Res 16:1152–1154

    Google Scholar 

  • Neale DB, Marshall KA, Sederoff RR (1989) Chloroplast and mitochondrial DNA are paternally inherited inSequoia sempervirens D. Don Endl. Proc Natl Acad Sci USA 86:9347–9349

    Google Scholar 

  • Neale DB, Marshall KA, Harry DE (1991) Inheritance of chloroplast and mitochondrial DNA in incense-cedar (Calocedrus decurrens). Can J For Res 21:717–720

    Google Scholar 

  • Newton KJ, Coe EH Jr, Gabay-Laughnan S, Laughnan JR (1989) Abnormal growth phenotypes and mitochondrial mutations in maize. Maydica 34:291–296

    Google Scholar 

  • Ohba K, Iwakawa M, Okada Y, Murai M (1971) Paternal transmission of a plastid anomaly in some reciprocal crosses of Sugi,Cryptomeria japonica D. Don. Silvae Genet 20:101–107

    Google Scholar 

  • Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–251

    Google Scholar 

  • Robertson D, Palmer JD, Earle ED, Mutschler MA (1987) Analysis of organelle genomes in a somatic hybrid derived from cytoplasmic male-sterileBrassica oleracea and atrazine-resistantB. campestris. Theor Appl Genet 74:303–309

    Google Scholar 

  • Rothenberg M, Boeshore ML, Hanson MR, Izhar S (1985) Intergenomic recombination of mitochondrial genomes in a somatic hybrid plant. Curr Genet 9:615–618

    Google Scholar 

  • Schnabel A, Asmussen MA (1989) Definition and properties of disequilibria within nuclear-mitochondrial-chloroplast and other nuclear-dicytoplasmic systems. Genetics 123:199–215

    Google Scholar 

  • Sederoff RR, Ronald P, Bedinger P, Rivin C, Walbot V, Bland M, Levings CS III (1986) Maize mitochondrial plasmid S-1 sequences share homology with chloroplast genepsbA. Genetics 113:469–482

    Google Scholar 

  • Smith SE (1988a) Biparental inheritance of organelles and its implications in crop improvement. Plant Breed Rev 6:361–393

    Google Scholar 

  • Smith SE (1988b) Influence of parental genotype on plastid inheritance inMedicago sativa. J Hered 80:214–217

    Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    Google Scholar 

  • Stern DB, Palmer JD (1984) Extensive and widespread homologies between mitochondrial and chloroplast DNA in plants. Proc Natl Acad Sci USA 81:1946–1950

    Google Scholar 

  • Stine M, Keathley DE (1990) Paternal inheritance of plastids in Engelmann spruce x blue spruce hybrids. J Hered 81:443–446

    Google Scholar 

  • Stine M, Sears BB, Keathley DE (1989) Inheritance of plastids in interspecific hybrids of blue spruce and white spruce. Theor Appl Genet 78:768–774

    Google Scholar 

  • Szmidt AE, Aldén T, Hällgren JE (1987) Paternal inheritance of chloroplast DNA inLarix. Plant Mol Biol 9:59–64

    Google Scholar 

  • Szmidt AE, El-Kassaby YA, Sigurgeirsson A, Aldén T, Lindgren D, Hällgren JE (1988) Classifying seedlots ofPicea sitchensis andP. glauca in zones of introgression using restriction analysis of chloroplast DNA. Theor Appl Genet 76:841–845

    Google Scholar 

  • Tilney-Bassett RAE, Almouslem AB (1989) Variation in plastid inheritance between pelargonium cultivars and their hybrids. Heredity 63:145–153

    Google Scholar 

  • Timmis JN, Scott NS (1983) Sequence homology between spinach nuclear and chloroplast genomes. Nature 305:65–67

    Google Scholar 

  • Wagner DB, Furnier GR, Saghai-Maroof MA, Williams SM, Dancik BP, Allard RW (1987) Chloroplast DNA polymorphisms in lodgepole and jack pines and their hybrids. Proc Natl Acad Sci USA 84:2097–2100

    Google Scholar 

  • Wagner DB, Govindaraju DR, Yeatman CW, Pitel JA (1989) Paternal chloroplast DNA inheritance in a diallel cross of jack pine (Pinus banksiana Lamb.). J Hered 80:483–485

    Google Scholar 

  • Wheeler NC, Guries RP (1987) A quantitative measure of introgression between lodgepole and jack pines. Can J Bot 65:1876–1885

    Google Scholar 

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Author notes

  1. J. Dong

    Present address: Jiangxi Agricultural University, Nanchang, Jiangxi Province, People's Republic of China

  2. A. D. Yanchuk

    Present address: British Columbia Ministry of Forests, Research Branch, 31 Bastion Square, Victoria, British Columbia, Canada

Authors and Affiliations

  1. Department of Forestry, University of Kentucky, 40546-0073, Lexington, KY, USA

    D. B. Wagner & J. Dong

  2. British Columbia Ministry of Forests, Kalamalka Research Station, 3401 Reservoir Road, V1B 2C7, Vernon, British Columbia, Canada

    M. R. Carlson

  3. Alberta Forest Service, Edmonton, Alberta, Canada

    A. D. Yanchuk

Authors
  1. D. B. Wagner
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  2. J. Dong
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  3. M. R. Carlson
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  4. A. D. Yanchuk
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Communicated by P. M. A. Tigerstedt

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Wagner, D.B., Dong, J., Carlson, M.R. et al. Paternal leakage of mitochondrial DNA inPinus . Theoret. Appl. Genetics 82, 510–514 (1991). https://doi.org/10.1007/BF00588607

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  • DOI: https://doi.org/10.1007/BF00588607

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