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Isolation and characterization of cDNA clones encodingcdc2 homologues fromOryza sativa: a functional homologue and cognate variants

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Summary

Using probes obtained by PCR amplification, we have isolated two cognate rice cDNAs (cdc2Os-1 andcdc2Os-2) encoding structural homologues of thecdc2 +/CDC28(cdc2) protein kinase from a cDNA library prepared from cultured rice cells. Comparison of the deduced amino acid sequences of cdc2Os-1 and cdc2Os-2 showed that they are 83 % identical. They are 62 % identical toCDC28 ofSaccharomyces cerevisiae and much more similar to the yeast and mammalian p34cdc2 kinases than to riceR2, acdc2-related kinase isolated previously by screening the same rice cDNA library with a different oligonucleotide probe. Southern blot analysis indicated that the three rice clones (cdc2Os-1,cdc2Os-2 andR2) are derived from distinct genes and are each found in a single copy per rice haploid genome. RNA blot analysis revealed that these genes are expressed in proliferating rice cells and in young rice seedlings.cdc2Os-1 could complement a temperature-sensitive yeast mutant ofcdc28. However, despite the similarity in structure, bothcdc2Os-2 andR2 were unable to complement the same mutant. Thus, the present results demonstrate the presence of structurally related, but functionally distinct cognates of thecdc2 cell cycle kinase in rice.

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Author notes

  1. Shingo Hata

    Present address: Faculty of Science, Himeji Institute of Technology, Kamigori, Ako-gun, 678-12, Hyogo, Japan

Authors and Affiliations

  1. National Institute of Agrobiological Resources, 305, Tsukuba, Ibaraki

    Junji Hashimoto, Toshio Hirabayashi, Yuriko Hayano, Shingo Hata & Yuko Ohashi

  2. National Institute of Animal Health, 305, Tsukuba, Ibaraki

    Iwao Suzuka

  3. Department of Biology, Faculty of Science, The University of Tokyo, 113, Bunkyoku, Tokyo, Japan

    Takahiko Utsugi, Akio Toh-E & Yoshiko Kikuchi

Authors
  1. Junji Hashimoto
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  2. Toshio Hirabayashi
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  4. Shingo Hata
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  5. Yuko Ohashi
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Additional information

Communicated by K. Isono

The nucleotide sequence data in this paper have been deposited in the EMBL database under accession number X60374 (cdc2Os-1) and X60375 (cdc2Os-2)

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Hashimoto, J., Hirabayashi, T., Hayano, Y. et al. Isolation and characterization of cDNA clones encodingcdc2 homologues fromOryza sativa: a functional homologue and cognate variants. Molec. Gen. Genet. 233, 10–16 (1992). https://doi.org/10.1007/BF00587555

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  • DOI: https://doi.org/10.1007/BF00587555

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