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A microelectrode for continuous monitoring of glucose concentration in isolated perfused tubule segments

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Abstract

The design and the application of a micro-enzyme-electrode for continuous monitoring of glucose concentration in the isolated tubule preparation is described. The principle of the electrode is the amperometric detection of hydrogen peroxide, which is a product of the oxidation ofd-glucose by glucose oxidase immobilized at the tip of a micro-electrode. The resulting current causes a voltage deflection across a resistor in series with the electrode that is correlated directly with the glucose concentration. The electrode response to glucose is almost linear over the concentration range from 0 to 12 mmol/l with a slightly diminished slope in the higher range. Other sugars (12 mmol/l raffinose, galactose, fructose, sucrose, mannitol), pH (from 6.5 to 8.0) andpCO2 (from 1 to 10 kPa) do not influence the reading. A reduction ofpO2 in the test solution to 1 kPa blunts the reading. Raising the temperature from 20°C to 40°C leads to a pronounced increase of the voltage deflection at a given glucose concentration. Interference is observed with strongly reducing agents such asl-cysteine, ascorbic acid and uric acid. At defined conditions the electrode is well suited to measure continuously glucose concentration in the luminal fluid at the collection site of the isolated perfused tubule of the kidney. Experiments are presented which illustrate the performance of the glucose electrode in this isolated tubule set-up. Peritubular reduction of potassium concentration or the application of ouabain diminish glucose reabsorption.

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Author notes

  1. Wolfgang Rehwald

    Present address: Institut für Medizinische Physik, Müllerstrasse 44, A-6020, Innsbruck, Austria

  2. Hans Oberleithner

    Present address: Physiologisches Institut der Universität, Röntgenring 9, D-8700, Würzburg, Germany

Authors and Affiliations

  1. Institut für Physiologie der Universität Innsbruck, Fritz-Pregl-Strasse 3, A-6010, Innsbruck, Austria

    Wolfgang Rehwald, John Geibel, Edith Gstrein & Hans Oberleithner

Authors
  1. Wolfgang Rehwald
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  2. John Geibel
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  3. Edith Gstrein
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  4. Hans Oberleithner
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Additional information

Supported by Österreichischer Forschungsfonds, Proj. No. 4366 Part of the data was presented at the 55th Meeting of the German Physiological Society, Innsbruck, 1981, Abstract. No. A228. 16th Annual Meeting of the European Society for Clinical Investigation, Luxembourg, 1982, Abstract No. R12. World Congress on Medical Physics and Biomedical Engineering, Hamburg, 1982, Abstract No. 7.03

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Rehwald, W., Geibel, J., Gstrein, E. et al. A microelectrode for continuous monitoring of glucose concentration in isolated perfused tubule segments. Pflugers Arch. 400, 398–402 (1984). https://doi.org/10.1007/BF00587539

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  • DOI: https://doi.org/10.1007/BF00587539

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