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Modifications of single and double-barrel potassium specific microelectrodes for physiological experiments

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Summary

  1. 1.

    The shape parameters of glass microcapillaries for making ion specific microelectrodes were studied. It was found, that the greater the angle set by the walls, the more advantageous is the shape for filling the microelectrode. The time response of the precisely prepared microelectrode does not exceed severall msec.

  2. 2.

    The preparation of the double-barrel ion specific microelectrodes is described. One barrel was filled with organic ion exchanger and the other with NaCl solution (reference channel). These electrodes are convenient for measurement of ion movements in the excitable tissues (brain, spinal cord etc) where it is necessary to avoid the electrical activity of the cells.

  3. 3.

    A side-pore type of ion specific microelectrode was developed for measurement of extracellular K+ concentration during muscle contraction. This type of electrode does not cut the muscle fibres during contractions and the side orifice of the channel prevents the undesirable movement of the ion exchanger during insertion into the muscle.

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Authors and Affiliations

  1. Institute of Physiology, Czechoslovak Academy of Sciences, Prague, Czechoslovakia

    F. Vyskočil & N. Kříž

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  1. F. Vyskočil
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  2. N. Kříž
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Vyskočil, F., Kříž, N. Modifications of single and double-barrel potassium specific microelectrodes for physiological experiments. Pflugers Arch. 337, 265–276 (1972). https://doi.org/10.1007/BF00586850

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  • DOI: https://doi.org/10.1007/BF00586850

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