Summary
Countertransport experiments on rings of everted jejunum of rats suggest that thiamine is actively transported by a mobile carrier in intestinal epithelial cells and that pyrithiamine, but not oxythiamine or deshydroxythiamine, has the common carrier with thiamine.
This is a preview of subscription content, log in via an institution to check access.
References
Agar, W. T., F. J. R. Hird, andG. S. Sidhu: The uptake of amino acids by the intestine. Biochim. biophys. Acta (Amst.)14, 80–84 (1954).
Alvarado, F.: The relationship between Na+ and the active transport of arbutin in the small intestine. Biochim. biophys. Acta (Amst.)109, 478 to 494 (1965).
Buchman, E. R., andE. M. Richardson: Thiamin analogs. II. 4-Methylthiazole analogs. J. Amer. chem. Soc.67, 395–399 (1945).
Cerecedo, L. R., S. Eich, andE. Bresnick: Effects of oxythiamine and neopyrithiamine on phosphorylation of thiamine by thiamine phosphorylase from rat intestine. Biochim. biophs. Acta (Amst.)15, 144–145 (1954).
Crane, R. K., andP. Mandelstam: The active transport of sugars by various preparations of hamster intestine. Biochim. biophys. Acta (Amst.)45, 460–476 (1960).
Fox, K. R., andC. A. M. Hogben: Active transport of nicotinic acid and thiamine by in vitro bullfrog small intestine. Fed. Proc.26, 502 (1967).
Johnson, L. R., andC. F. Gubler: Studies on the physiological functions of thiamine. III. The phosphorylation of thiamine in brain. Biochim. biophys. Acta (Amst.)156, 85–96 (1968).
Kishi, H., andE. Hiraoka: Studies on deoxythiamine. V. The effects of deoxythiamine on enzymes related to thiamine. Vitamins (Japan)35, 146–150 (1967).
Krebs, H. A., u.K. Henseleit: Untersuchungen über die Harnstoffbildung im Tierkörper. Hoppe-Seylers Z. physiol. Chem.210, 33–66 (1932).
Mano, Y., andR. Tanaka: Studies on enzymatic synthesis of cocarboxylase in animal tissue. IV. Effect of metallic ions, various nucleotides and thiamine derivatives on thiaminokinase from rat liver. J. Biochem.47, 401–413 (1960).
Rindi, G., andU. Ventura: Phosphorylation and uphill intestinal transport of thiamine, in vitro. Experientia (Basel)23, 175–176 (1967).
Rydon, H. N.: Note on an improved method for the preparation of oxythiamine. Biochem. J.48, 383–384 (1951).
Sandner, E., u.B. Gassmann: Über den Einfluß von Verdauungssäften auf die Phosphorylierung und Resorption des Vitamin B1. Die Nahrung11, 583–594 (1967).
Sharma, S. K., andY. H. Quastel: Transport and metabolism of thiamine in rat brain cortex in vitro. Biochem. J.94, 790–800 (1965).
Stein, W. D.: The movement of molecules across cell membranes. New York: Academic Press 1967.
Ventura, U., andG. Rindi: Transport of thiamine by the small intestine in vitro. Experientia (Basel)21, 645–646 (1965).
Wilbrandt, W., andT. Rosenberg: The concept of carrier transport and its corollaries in pharmacology. Pharmacol. Rev.13, 109–183 (1961).
Wilson, T. H., andG. Wiseman: The use of sacs of everted small intestine for the study of the transference of substances from the mucosal to the serosal surface. J. Physiol. (Lond.)123, 116–125 (1954).
Author information
Authors and Affiliations
Additional information
This investigation was partially supported by a grant (No. 115. 2162. O 1323) from Consiglio Nazionale delle Ricerche, Rome, Italy.
Rights and permissions
About this article
Cite this article
Rindi, G., Ventura, U. Thiamine Countertransport in rat small intestine. Pflugers Arch. 310, 185–188 (1969). https://doi.org/10.1007/BF00586775
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00586775