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Balance between tubular flow rate and net fluid reabsorption in the proximal convolution of the rat kidney

I. Dependency of reabsorptive net fluid flux upon proximal tubular surface area at spontaneous variations of filtration rate

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Summary

In rats with hereditary Diabetes insipidus (Brattleboro strain) proximal fluid reabsorption was studied during spontaneous variations of GFR. Independent of kidney weight and arterial blood pressure total kidney GFR varied between 0.35 ml/min×gKW and 1.8 ml/min×gKW, while single nephron filtrate showed a variation between 18 nl/min×gKW and 66 nl/min×gKW. End-proximal TF/P inulin of 78 fluid samples did not correlate with filtration rate and averaged 2.38±0.55 S.D. Also, proximal transit time was independent of filtration rate with a mean of 9.7 sec±1.1 S.D. These results imply a direct correlation between mean luminal radius and filtration rate, which was demonstrated by microphotography of the renal surface.

Our data show that complete glomerulo-tubular balance in the proximal tubule exists during spontaneous variations of glomerular filtration rate. In support of the hypothesis ofGertz [4,6] the lateral net fluid flux was found to be linearly dependent upon tubular surface area. In addition, intratubular volume changed in direct proportion to single nephron filtrate. The present results are interpreted to further strengthen the assumption of a critical influence of tubular geometry on proximal fluid reabsorption during spontaneous variations of GFR.

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References

  1. Brunner, F. P., F. C. Rector, andD. W. Seldin: Mechanism of glomerulotubular balance. II. Regulation of proximal tubular reabsorption by tubular volume, as studied by stopped-flow microperfusion. J. clin. Invest.45, 603 (1966).

    Google Scholar 

  2. Davis, B. B., F. G. Knox, andR. W. Berliner: The effect of vasopressin on proximal tubule sodium reabsorption in the dog. Amer. J. Physiol.212, 1361 (1967).

    Google Scholar 

  3. Führ, J., J. Kaczmarczyk u.C. D. Krüttgen: Eine einfache Methode zur Inulinbestimmung für Nieren-Clearance-Untersuchungen bei Stoffwechselgesunden und Diabetikern. Klin. Wschr.33, 729 (1955).

    Google Scholar 

  4. Gertz, K. H.: Transtubuläre Natriumchloridflüsse und Permeabilität für Nichtelektrolyte im proximalen und distalen Konvolut der Rattenniere. Pflügers Arch. ges. Physiol.276, 336 (1963).

    Google Scholar 

  5. —,G. C. Kennedy u.K. J. Ullrich: Mikropunktionsuntersuchungen über die Flüssigkeitsrückresorption aus einzelnen Tubulusabschnitten bei Wasserdiurese (Diabetes insipidus). Pflügers Arch. ges. Physiol.278, 513 (1964).

    Google Scholar 

  6. —,J. A. Mangos, G. Braun, andH. D. Pagel: On the glomerular tubular balance in the rat kidney. Pflügers Arch. ges. Physiol.285, 360 (1965).

    Google Scholar 

  7. Giebisch, G., andE. E. Windhager: Renal tubular transfer of sodium, chloride and potassium. Amer. J. Med.36, 643 (1964).

    Google Scholar 

  8. Glabman, S., H. S. Aynedjian, andN. Bank: Micropuncture study of the effect of acute reductions in glomerular filtration rate on sodium and water reabsorption by the proximal tubules of the rat. J. clin. Invest.44, 1410 (1965).

    Google Scholar 

  9. Hierholzer, K., M. Wiederholt u.H. Stolte: Hemmung der Natriumresorption im proximalen und distalen Konvolut adrenalektomierter Ratten. Pflügers Arch. ges. Physiol.291, 43 (1966).

    Google Scholar 

  10. Hilger, H. H., J. D. Klümper u.K. J. Ullrich: Wasserrückresorption und Ionentransport durch die Sammelrohrzellen der Säugetierniere. Pflügers Arch. ges. Physiol.267, 218 (1958).

    Google Scholar 

  11. Horster, M., andK. Thurau: Micropuncture studies on the filtration rate of single superficial and juxtamedullary glomeruli in the rat kidney. Pflügers Arch. ges. Physiol.301, 162 (1968).

    Google Scholar 

  12. Kelman, R. B.: A theoretical note on exponential flow in the proximal part of the mammalian nephron. Bull. Math. Biophys.24, 303 (1962).

    Google Scholar 

  13. Landwehr, D. M., R. M. Klose, andG. Giebisch: Renal tubular sodium and water reabsorption in the isotonic sodium chloride loaded rat. Amer. J. Physiol.212, 1327 (1967).

    Google Scholar 

  14. Landwehr, D. M., J. Schnermann, R. M. Klose, andG. Giebisch: The effect of acute reduction in glomerular filtration rate on renal tubular sodium and water reabsorption. Amer. J. Physiol. (in press).

  15. Levine, D. Z., G. Liebau, H. Fischbach, andK. Thurau: Micropuncture studies in the dog kidney. II. Reabsorptive characteristics of the proximal tubule during spontaneous and experimental variations in GFR and during drug induced natriuresis. Pflügers Arch. (in press).

  16. Lewy, J. E., andE. E. Windhager: Peritubular control of proximal tubular fluid reabsorption in the rat kidney. Amer. J. Physiol.214, 943 (1968).

    Google Scholar 

  17. Leyssac, P. P.: Dependence of glomerular filtration rate on proximal tubular reabsorption of salt. Acta physiol. scand.58, 236 (1963).

    Google Scholar 

  18. Rector, F. C., F. P. Brunner, andD. W. Seldin: Mechanism of glomerulotubular balance. I. Effect of aortic constriction and elevated ureteropelvic pressure on glomerular filtration rate, fractional reabsorption, transit time, and tubular size in the proximal tubule of the rat. J. clin. Invest.45, 590 (1966).

    Google Scholar 

  19. —,J. C. Sellman, M. Martinez-Maldonado, andD. W. Seldin: The mechanism of suppression of proximal tubular reabsorption by saline infusions. J. clin. Invest.46, 47 (1967).

    Google Scholar 

  20. Schnermann, J., H. Valtin, K. Thurau, W. Nagel, M. Horster, H. Fischbach, M. Wahl, andG. Liebau: Influence of antidiuretic hormone on tubular net fluid reabsorption in rats with hereditary hypothalamic Diabetes insipidus. Pflügers Arch. (in press).

  21. Steinhausen, M.: Eine Methode zur Differenzierung proximaler und distaler Tubuli der Nierenrinde von Ratten in vivo und ihre Anwendung zur Bestimmung tubulärer Strömungsgeschwindigkeiten. Pflügers Arch. ges. Physiol.277, 23 (1963).

    Google Scholar 

  22. —: Messungen des tubulären Harnstromes und der tubulären Reabsorption unter erhöhtem Ureterdruck. Intravitalmikroskopische Untersuchungen an der Nierenrinde von Ratte. Pflügers Arch. ges. Physiol.298, 105 (1967).

    Google Scholar 

  23. —,A. Loreth u.S. Olson: Messungen des tubulären Harnstromes, seine Beziehungen zum Blutdruck und zur Inulin-Clearance. Pflügers Arch. ges. Physiol.286, 118 (1965).

    Google Scholar 

  24. Thurau, K., u.P. Deetjen: Kinematographische Untersuchungen am Warmblüternephron. Nachr. Akad. Wissensch. Göttingen2, 27 (1961).

    Google Scholar 

  25. Valtin, H., H. A. Schroeder, K. Benirschke, andH. W. Sokol: Familial hypothalamic diabetes insipidus in rats. Nature (Lond.)196, 1109 (1962).

    Google Scholar 

  26. Wahl, M., W. Nagel, H. Fischbach, andK. Thurau: On the application of the occlusion time method for measurements of lateral net fluxes in the proximal convolution of the rat kidney. Pflügers Arch. ges. Physiol.298, 141 (1967).

    Google Scholar 

  27. Wiederholt, M., K. Hierholzer, E. E. Windhager, andG. Giebisch: Microperfusion study of fluid reabsorption in proximal tubules of rat kidney. Amer. J. Physiol.213, 809 (1967).

    Google Scholar 

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Authors and Affiliations

  1. Physiologisches Institut der Universität München, München, Germany

    J. Schnermann, M. Wahl, G. Liebau & H. Fischbach

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  1. J. Schnermann
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  2. M. Wahl
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  3. G. Liebau
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  4. H. Fischbach
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Supported by the Deutsche Forschungsgemeinschaft and the U.S. Department of the Army, through its European Research Office.

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Schnermann, J., Wahl, M., Liebau, G. et al. Balance between tubular flow rate and net fluid reabsorption in the proximal convolution of the rat kidney. Pflugers Arch. 304, 90–103 (1968). https://doi.org/10.1007/BF00586722

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  • DOI: https://doi.org/10.1007/BF00586722

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