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Fluorometrisch-enzymatische Mikround ultramikrobestimmung von Inulin und Glucose

Fluorometric-Enzymatic determination of inulin and glucose in the micro and ultramicro range

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Summary

Enzymatic methods of inulin and glucose determination are adapted to sample sizes in the micro-and ultramicro range and modified for fluorometric measurements. Using a highly sensitive ultramicro method inulin and/or glucose could be measured exactly in concentrations of 5 mgm per cent in samples of 1 nl (1×10−9 l) corresponding to absolute amounts of 1×10−12 gm.

Zusammenfassung

Es werden verschiedene fluorometrische Modifikationen der enzymatischen Inulin-und Glucosebestimmung im Mikro-und Ultramikrobereich beschrieben. Bei Anwendung der empfindlichen Ultramikrobestimmung ist in einer Probenmenge von 1 nl (1·10−9 l) eine Inulin-bzw. Glucosekonzentration von 5 mg% oder ein absoluter Gehalt von 1·10−12 g Inulin bzw. Glucose meßbar.

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Literatur

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Authors and Affiliations

  1. Max-Planck-Institut für Biophysik, Frankfurt am Main

    R. Zwiebel, B. Höhmann, P. Frohnert & K. Baumann

Authors
  1. R. Zwiebel
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  2. B. Höhmann
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  3. P. Frohnert
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  4. K. Baumann
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Ich danke der Deutschen Forschungsgemeinschaft für ein Ausbildungsstipendium.

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Zwiebel, R., Höhmann, B., Frohnert, P. et al. Fluorometrisch-enzymatische Mikround ultramikrobestimmung von Inulin und Glucose. Pflugers Arch. 307, 127 (1969). https://doi.org/10.1007/BF00586469

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  • DOI: https://doi.org/10.1007/BF00586469

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