Summary
The main excretory duct of the rabbit submaxillary gland was isolated and studied in a lucite perfusion chamber. Measurements of transepithelial electrical potential difference (p.d.) and specific electrical wall resistance (R m) were performed and short-circuit-current (SCC) was determined under conditions of different test solutions placed at the blood side of the duct.
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1.
High molecular compounds such as albumin or dextran are required in the bathing medium to maintain transport function.
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2.
Active transport of sodium from the lumen to the interstitium can be maintained only when there is both pyruvate and acetate present in a bicarbonate Ringer's bathing medium. Isocitrate can substitute for pyruvate and acetate. SCC was found to be 1.7±0.2 mA/cm2 with the artificial bathing fluid as compared to 1.9±0.2 mA/cm2 with rabbit serum placed at the blood side of the duct.
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3.
In the absence of bicarbonate Na+ transport is reduced to 55±10% of control.
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4.
Potassium in a 4 mM concentration on the blood side proved to be indispensable for transport function. In the absence of interstitial potassium the Na+ conductance of the luminal cell membrane is reduced. Rubidium and cesium ions can partially substitute for potassium.
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With technical assistance of B. Gebler.
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Knauf, H. The minimum requirements for the maintenance of active sodium transport across the isolated salivary duct epithelium of the rabbit. Pflugers Arch. 333, 326–336 (1972). https://doi.org/10.1007/BF00586212
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DOI: https://doi.org/10.1007/BF00586212