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Differential effects of ouabain and ethacrynic acid on the labyrinthine potentials

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Summary

Intravenous injection of 20–45 mg/kg Ethacrynic acid reversibly reduced the endocochlear potential and increased endolymph sodium concentration from less than 1 mM to 5–8 mM 20 min after the injection.

Intravenous injection of 42–66 mg/kg Ethacrynic acid drastically reduced the endocochlear potential to negative values while the utricular potential remained unaffected. However perfusion of 1×10−3 M Ethacrynic acid into the perilymphatic space reduced both potentials at approximately the same rate, indicating that the differential effect of Ethacrynic acid on the endocochlear and utricular potentials, was due to differences in vascularity and not to differences in the action of Ethacrynic acid.

The maximum negative potential produced by intravenous Ethacrynic acid in the cochlea and by perilymphatic application in both the cochlea and utricle was rapidly reduced by anoxia without changes in the rate of influx of sodium.

A similar anoxia sensitive negative potential was not produced by the application of ouabain in either the utricle or cochlea even though both potentials were reduced to negative values. Inclusion of 1×10−3 M ouabain into the 1×10−3 M Ethacrynic acid perilymph perfusate reduced the anoxia sensitive negative potential by 50 to 80% in the utricle and cochlea respectively.

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References

  • Baba, W. I., Smith, A. J., Townshend, Mary M.: A comparison of the effects of ethacrynic acid and a mercurial diuretic (Mersalyl) on sodium transport across the isolated frog skin. Brit. J. Pharmacol. Chemother.28, 238–245 (1966)

    Google Scholar 

  • Bosher, S. K., Smith, C., Warren, R. L.: The effects of ethacrynic acid upon the cochlear endolymph and the stria vascularis. A preliminary report. Acta otolaryng., (Stockh.)75, 184–191 (1973)

    Google Scholar 

  • Cohn, E. S., Gordes, E. H., Brusilow, S. W.: “Ethacrynic acid effect on the composition of cochlear fluids”. Science171, 910–911 (1971)

    Google Scholar 

  • Davis, P. W.: Inhibition of renal Na+, K+-activated adenosine triphosphatase activity by ethacrynic acid. Biochem. Pharmacol.19, 1983–1989 (1970)

    Google Scholar 

  • Duggan, D. E., Noll, R. M.: Effects of ethacrynic acid and cardiac glycosides upon a membrane ATPase of renal cortex. Arch. Biochem. Biophys.109, 388–396 (1965)

    Google Scholar 

  • Dunn, M. J.: The effects of transport inhibitors on sodium outflux and influx in red blood cells: evidence for exchange diffusion. J. clin. Invest.49, 1804–1814 (1970)

    Google Scholar 

  • Gordon, E. E.: Site of ethacrynic acid action on Ehrlich ascites tumor, cells. Biochem. Pharmacol.17, 1237–1242 (1968)

    Google Scholar 

  • Gordon, E. E., Hartog, M. de: The relationship between cell membrane potassium ion transport and glycolysis. J. gen. Physiol.54, 650–663 (1969)

    Google Scholar 

  • Hanzelik, K., Peppercorn, M.: Deafness after ethacrynic acid. Lancet1969, 416

  • Hoffman, J. F., Kregenow, F. M.: the characterization of new energy-dependent cation transport processes in red blood cells. Ann. N.Y. Acad. Sci.137, 566–576 (1966)

    Google Scholar 

  • Hook, J. B., Williamson, H. E.: Lack of correlation between natriuretic activity and inhibition of renal Na−K-activated ATPase. Proc. Soc. exp. Biol. (N.Y.)120, 358–360 (1965)

    Google Scholar 

  • Kernan, R. P.: Membrane potential changes during sodium transport in frog sartorius muscle. Nature (Lond.)193, 986 (1962)

    Google Scholar 

  • Klahr, S., Yates, J., Bourgoignie, J.: Inhibition of glycolysis by ethacrynic acid and furosemide. Amer. J. Physiol.221, 1038–1043 (1971)

    Google Scholar 

  • Kuijpers, W., Bonting, S. L.: The cochlear potentials. II. The nature of the cochlear endolymphatic resting potential. Pflügers Arch.320, 359–372 (1970)

    Google Scholar 

  • Kusakari, J., Thalman, R.: Effect of ethacrynic acid upon energy utilization in the cochlea. Proceedings of the 85th meeting of the Acoustical Society of America (1973)

  • Lubowitz, H., Whittam, R.: Ion movements in human red cells independent of the sodium pump. J. Physiol. (Lond.)202, 111–131 (1969)

    Google Scholar 

  • Maher, J. F., Schreiner, G. E.: Studies on ethacrynic acid in patients with refractory edema. Ann. intern. Med.62, 15–29 (1965)

    Google Scholar 

  • Mathog, R. H., Thomas, W. G., Hudson, W. R.: Ototoxicity of new and potent diuretics. Arch. Otolaryng. 7–13 (1970)

  • Matz, J. G., Beal, D. D., Krames, L.: Ototoxicity of ethacrynic acid. Arch. Otolaryng.90, 152–155 (1969)

    Google Scholar 

  • Nechay, B. R., Palmer, R. F., Chinoy, D. A., Posey, V. A.: The problem of Na+ +K+ adenosine triphosphatase as the receptor for diuretic action of mercurials and ethacrynic acid. J. Pharmacol. Ther.157, 599–617 (1967)

    Google Scholar 

  • Petersen, O. H.: Some factors influencing stimulation-induced release of potassium from the cat sub-mandibular gland fluid perfused through the gland. J. Physiol. (Lond.)208, 437–447 (1970)

    Google Scholar 

  • Pillay, V. K. G., Schwartz, F. D., Aimi, K., Kark, R. M.: Transient and permanent deafness following treatment with ethacrynic acid in renal failure. Lancet1969, 77–79

  • Prazma, J., Thomas, W. G., Fischer, N. D., Preslar, M. J.: Ototoxicity of ethacrynic acid. Arch. Otolaryng.95, 448–456 (1972)

    Google Scholar 

  • Quick, C. A., Duvall, A. J.: Early changes in the cochlear duct from ethacrynic acid: an electronmicroscopic evaluation. Laryngoscope (St. Louis)80, 954–965 (1970)

    Google Scholar 

  • Rang, H. P., Ritchie, J. M.: On the electrogenic sodium pump in mammalian nonmyelinated nerve fibres and its activation by various external cations. J. Physiol. (Lond.)196, 183–221 (1968)

    Google Scholar 

  • Rettori, O., Lenoir, J. P.: Ouabain-insensitive active sodium transport in erythrocytes: effect of external cation. Amer. J. Physiol.222, 880–884 (1972)

    Google Scholar 

  • Schneider, W. J., Becker, E. L.: Acute transient hearing loss after ethacrynic acid therapy. Arch. intern. Med.117, 715–717 (1966)

    Google Scholar 

  • Sellick, P. M., Johnstone, B. M.: Changes in cochlear endolymph Na+ concentration measured with Na+ specific microelectrodes. Pflügers Arch.336, 11–20 (1972)

    Google Scholar 

  • Sellick, P. M., Johnstone, J. R., Johnstone, B. M.: The electrophysiology of the utricle. Pflügers Arch.336, 21–27 (1972)

    Google Scholar 

  • Silverstein, H., Yules, R. B.: The effect of diuretics on cochlear potentials and inner ear fluids. Laryngoscope (St. Louis)81, 873–888 (1971)

    Google Scholar 

  • Smith, C. A.: Microscopic structure of the utricle. Ann. Otol.65, 450–469 (1956)

    Google Scholar 

  • Whittembury, G.: Na and water transport in kidney proximal tubular cells. J. gen. Physiol.51, 303s-314s (1968)

    Google Scholar 

  • Whittembury, G., Fishman, J.: Relation between cell Na extrusion and transtubular absorption in the perfused toad kidney; the effect of K, ouabain and ethacrynic acid. Pflügers Arch.307, 138 (1969)

    Google Scholar 

  • Whittembury, G., Proverbio, F.: Two modes of Na extrusion in cells from guinea pig kidney cortex slices. Pflügers Arch.316, 1–25 (1969)

    Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, University of Western Australia, Nedlands, Australia

    Peter M. Sellick & Brian M. Johnstone

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  1. Peter M. Sellick
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  2. Brian M. Johnstone
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Sellick, P.M., Johnstone, B.M. Differential effects of ouabain and ethacrynic acid on the labyrinthine potentials. Pflugers Arch. 352, 339–350 (1974). https://doi.org/10.1007/BF00585686

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  • DOI: https://doi.org/10.1007/BF00585686

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