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A microscope stage temperature controller for the study of whole-cell or single-channel currents

  • Excitable Tissues and Central Nervous Physiology
  • Instruments and Techniques
  • Published:
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Abstract

The construction of a microscope stage temperature controller is described that works equally well with upright or inverted microscopes. The control circuit directly regulates the bath temperature near the physiological preparation from 0.0°C to 40.0°C with a stability of ±0.1°C; it allows biophysical studies of whole-cell or single channel currents to be carried out at different temperatures without introducing additional electrical noise into the measurements. The device may also be of interest to neurobiologists who work with tissue-culture preparations.

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Author notes

  1. Lee D. Chabala

    Present address: Department of Physiology and Biophysics, Cornell University Medical College, 10021, New York, New York, USA

  2. Robert E. Sheridan

    Present address: Department of Pharmacology, Georgetown University School of Medicine, 20007, Washington, D.C, USA

Authors and Affiliations

  1. Division of Biology, California Institute of Technology, 91125, Pasadena, California, USA

    Lee D. Chabala, Robert E. Sheridan, David C. Hodge, John N. Power & Michael P. Walsh

Authors
  1. Lee D. Chabala
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  2. Robert E. Sheridan
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  3. David C. Hodge
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  4. John N. Power
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  5. Michael P. Walsh
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Chabala, L.D., Sheridan, R.E., Hodge, D.C. et al. A microscope stage temperature controller for the study of whole-cell or single-channel currents. Pflugers Arch. 404, 374–377 (1985). https://doi.org/10.1007/BF00585351

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  • DOI: https://doi.org/10.1007/BF00585351

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