Abstract
Volume flow (J v), potential difference (ΔΨ), shortcircuit current (i 0) and electrical resistance (R) were measured simultaneously across bovine tracheal epithelium in vitro. Under basal conditions, with no applied hydrostatic or osmotic pressure gradient (ΔP=0, ΔΠ=0), no spontaneousJ v was observed. ΔΨ was 31±2 mV (lumen negative),i 0 161±8 μA cm−2 andR 202±9 Ω cm2,n=50. When a ΔΠ was applied, by adding 20–80 mM sucrose into the medium bathing either the luminal or the serosal side of the tissue, a linear relationship was found between ΔΠ andJ v toward the lumen or toward the serosa. The apparent hydraulic conductivity (apparentL p) was 4.6–4.910−6 cm s−1 atm−1. Histamine 10−4 M did not induce any spontaneousJ v under basal conditions and had no effect oni 0 nor onR. However, histamine caused a 100% increase inJ v elicited by sucrose gradients. It was concluded that histamine exerts a selective action on the hydraulic conductivity of bovine tracheal epithelium. Experiments using H1-receptors antagonists (diphenhydramine, dimetindene, chloropyramine) and H2-antagonists (cimetidine, metiamide) or a H2-agonist (impromidine) showed that the increase ofL p induced by histamine was mediated via H2-receptors.
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Supported by the Swiss National Foundation (SNF), grant no. 3.5880.79
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Durand, J., Durand-Arczynska, W. & Haab, P. Volume flow, hydraulic conductivity and electrical properties across bovine tracheal epithelium in vitro: Effect of histamine. Pflugers Arch. 392, 40–45 (1981). https://doi.org/10.1007/BF00584580
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DOI: https://doi.org/10.1007/BF00584580