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Choline acetyltransferase in the heart of adult rats

  • Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology
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Abstract

The distribution of choline acetyltransferase (ChAT, EC 2.3.1.6) in the heart of adult rats has been reinvestigated in view of recent discoveries that acetylcholine (ACh) can be synthesized not only by ChAT, but also by carnitine acetyltransferase (CarAT, EC 2.3.1.7) and that it is possible to distinguish between the ACh-synthesizing activity of ChAT in intramuscular nerves and the CarAT-mediated extraneural synthesis of ACh by means of bromoacetylcholine (BrACh), a specific inhibitor of ChAT. BrACh (0.002 mmol/l) has been found to inhibit the synthesis of ACh in the atria by 66–85% and in the ventricles by only 19–29%. Bromoacetylcarnitine (BrACar, 0.02 mmol/l), an inhibitor of CarAT, inhibited the synthesis of ACh in the atria by 34% and in the ventricles by 74–80%. These findings indicate that ChAT is responsible for most of the synthesis of ACh observed in the homogenates of the atria; in the ventricles, it catalyses only a minor portion of the total ACh synthesis observed. In the investigation of the regional distribution of ChAT in the heart, the BrACh-sensitive part of ACh synthesis was taken as the measure of ChAT activity. The highest activity of ChAT (nmol ACh synthesized ·g−1·h−1) was found in the region of the sinoatrial aode (1775); it decreased in the order: interatrial septum (781) > rest of the right atrium (712) > left atrium (416) > basal part of the right ventricle (366) > apical part of the right ventricle (250) > inter-ventricular septum (239) > basal and apical part of the left ventricle (208 and 205). The results indicate that earlier investigations of the distribution of ChAT in the heart provided a basically correct picture although the contribution of CarAT to the synthesis of ACh measured had not been excluded, and confirm that ChAT is present throughout the heart, including the apical parts of the ventricles. However, the sino-atrio-ventricular gradient of ChAT distribution is steeper when the contribution of CarAT to the synthesis of ACh is excluded.

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References

  • Brown O (1976) Cat heart acetylcholine: structural proof and distribution. Am J Physiol 231:781–785

    Google Scholar 

  • Brown O (1981) Cat heart acetylcholine: unilateral vagotomy studies with pyrolysis-mass fragmentography. Life Sci 28:819–825

    Google Scholar 

  • Burn JH, Milton AS (1959) Choline acetylase activity in the atria and its possible relation to the maintenance of the membrane potential. Br J Pharmacol 14:493–496

    Google Scholar 

  • Chase JFA, Tubbs PK (1970) Specific alkylation of a histidine residue in carnitine acetyltransferase by bromoacetyl-L-carnitine. Biochem J 116:713–720

    Google Scholar 

  • Comline RS (1946) Synthesis of acetylcholine by non-nervous tissue. J Physiol 105:6–7P

    Google Scholar 

  • Dlouhá J, Teisinger J, Vyskočil F (1979) Activation of membrane Na+/K+-ATPase of mouse skeletal muscle by acetylcholine and its inhibition by α-bungarotoxin, curare and atropine. Pflügers Arch 380:101–104

    Google Scholar 

  • Ekström J (1970) Distribution of choline acetyltransferase in the hearts of mammals. Acta Physiol Scand 80:73–78

    Google Scholar 

  • Ekström J (1974) Choline acetyltransferase in the heart and salivary glands of the rat after physical training. Q J Exp Physiol 59:73–80

    Google Scholar 

  • Fields JZ, Roeske WR, Morkin E, Yamamura HI (1978) Cardiac muscarinic cholinergic receptors. Biochemical identification and characterization. J Biol Chem 253:3251–3258

    Google Scholar 

  • Fonnum F (1969a) Isolation of choline esters from aqueous solutions by extraction with sodium tetraphenylboron in organic solvents. Biochem J 113:291–298

    Google Scholar 

  • Fonnum F (1969b) Radiochemical micro assays for the determination of choline acetyltransferase and acetylcholinesterase activities. Biochem J 115:465–472

    Google Scholar 

  • Fritz IB, Schultz SK (1965) Carnitine acetyltransferase. II. Inhibition by carnitine analogues and by sulfhydryl reagents. J Biol Chem 240:2188–2196

    Google Scholar 

  • Gerebtzoff MA (1959) Cholinesterases. A histochemical contribution to the solution of some functional problems. Pergamon Press, London, pp 132–134

    Google Scholar 

  • Gutkin VI (1962) The effect of depancreatization upon chemical components of the cholinergic system. (In Russian) Fiziol Zh SSSR 48:1085–1090

    Google Scholar 

  • Henderson GI, Sastry BVR (1978) Human placental choline acetyltransferase. Nature and molecular aspects of the inhibition by iodo- and bromoacetylcholines. Biochem Pharmacol 27:1331–1340

    Google Scholar 

  • Higgins CB, Vatner SF, Braunwald E (1973) Parasympathetic control of heart. Pharmacol Rev 25:119–155

    Google Scholar 

  • Jacobowitz D, Cooper T, Barner HB (1967) Histochemical and chemical studies of the localization of adrenergic and cholinergic nerves in normal and denervated cat hearts. Circ Res 20:289–298

    Google Scholar 

  • Koudelková Z, Vlk J (1962) The contents and distribution of acetylcholine in the heart auricles of the rat in experimental hyperthyreosis. (In Czech.) Plzeň Lék Sbor 19:75–78

    Google Scholar 

  • Lund DD, Schmid PG, Kelley SE, Corry RJ, Roskoski R (1978) Choline acetyltransferase activity in rat heart after transplantation. Am J Physiol 235:H367-H371

    Google Scholar 

  • Lund DD, Schmid PG, Roskoski R (1979) Choline acetyltransferase activity in rat and guinea pig heart following vagotomy. Am J Physiol 236:H620-H623

    Google Scholar 

  • Marvin WJ, Hermsmeyer K, McDonald RI, Roskoski LM, Roskoski R (1980) Ontogenesis of cholinergic innervation in the rat heart. Circ Res 46:690–695

    Google Scholar 

  • Mazel P, Holland C (1958) Acetylcholine and electrolyte metabolism in the various chambers of the frog and turtle heart. Circ Res 6:684–688

    Google Scholar 

  • McCaman RE, McCaman MW, Stafford ML (1966) Carnitine acetyltransferase in nervous tissue. J Biol Chem 241:930–934

    Google Scholar 

  • Milton AS (1959) The enzyme systems concerned with acetylcholine synthesis in the atria of the rabbit heart. J Physiol 49:462–470

    Google Scholar 

  • Molenaar PC, Polak RL (1980) Acetylcholine synthesizing enzymes in frog skeletal muscle. J Neurochem 36:1021–1025

    Google Scholar 

  • Morris D, Grewaal DS (1969) Halogen substituted derivatives of acetylcholine as inhibitors of choline acetyltransferase. Life Sci 8:511–516

    Google Scholar 

  • Morris D, Grewaal DS (1971) Human placental choline acetyltransferase. Eur J Biochem 22:563–572

    Google Scholar 

  • Roskoski R (1974) Choline acetyltransferase: Reversible inhibition by bromoacetyl coenzyme A and bromoacetylcholine. Biochemistry 13:2295–2298

    Google Scholar 

  • Roskoski R, Mayer HE, Schmid PG (1974) Choline acetyltransferase activity in guinea-pig heart in vitro. J Neurochem 23:1197–1200

    Google Scholar 

  • Roskoski R, Schmid PG, Mayer HE, Abboud FM (1975) In vitro acetylcholine biosynthesis in normal and failing guinea pig hearts. Cire Res 36:547–552

    Google Scholar 

  • Roskoski R, McDonald RI, Roskoski L, Marvin WJ, Hermsmeyer K (1977) Choline acetyltransferase activity in heart: evidence for neuronal and not myocardial origin. Am J Physiol 233:H642-H646

    Google Scholar 

  • Rothschuh KE (1954) Das herzmuskeleigene Acetylcholin. II. Der normale Acetylcholin-Gehalt der Vorhofs-und Kammermuskulatur beim Frosch und bei der Ratte. Pflügers Arch 258:481–488

    Google Scholar 

  • Rothschuh KE (1955) Das herzmuskeleigene Acetylcholin. III. Der Einfluß von Äthernarkose, O2-Mangel und Hemmung der Acetylcholinesterase auf den Acetylcholingehalt des Herzens. Pflügers Arch 260:374–384

    Google Scholar 

  • Rothschuh KE, Berkel HA (1955) Das herzmuskeleigene Acetylcholin. IV. Der Acetylcholingehalt des Ratten- und Meerschweinchenherzens unter dem Einfluß der Vagusreizung. Pflügers Arch 261:24–31

    Google Scholar 

  • Sastry BVR, Sadavongvivad C (1979) Cholinergic systems in nonnervous tissues. Pharmacol Rev 30:65–132

    Google Scholar 

  • Stanley RL, Conatser J, Dettbarn W-D (1978) Acetylcholine, choline acetyltransferase and cholinesterases in the rat heart. Biochem Pharmacol 27:2409–2411

    Google Scholar 

  • Tcheng KT (1951) Innervation of the dog's heart. Am Heart J 41:512–524

    Google Scholar 

  • Tuček S (1964) The distribution of choline acetylase in the cardiac auricles of rats, rabbits, cats and guinea-pigs. Physiol Bohemoslov 13:39–47

    Google Scholar 

  • Tuček S (1965) Changes in choline acetyltransferase activity in the cardiac auricles of dogs during postnatal development. Physiol Bohemoslov 14:530–535

    Google Scholar 

  • Tuček S (1973) An improved solvent system for liquid scintillation counting in choline acetyltransferase activity assays by Fonnum's method. J Neurochem 20:901–903

    Google Scholar 

  • Tuček S (1978) Acetylcholine synthesis in neurons. Chapman and Hall, London

    Google Scholar 

  • Tuček S (1981a) Effects of bromoacetylcholine and bromoacetylcarnitine on the synthesis of acetylcholine in the peripheral nerve and skeletal muscle. Physiol Bohemoslov 30:464

    Google Scholar 

  • Tuček S (1982) The synthesis of acetylcholine in skeletal muscles of the rat. J Physiol (Lond) 322 (in press)

  • Vlk J (1957) Über den Einfluß von Chlorpromazin auf den Acetylcholingehalt des Herzens. Naunyn-Schmiedebergs Arch Exp Path Pharmak 231:591–592

    Google Scholar 

  • Vlk J (1958a) Zur Frage des herzmuskeleigenen Acetylcholins. Naunyn-Schmiedebergs Arch Exp Path Pharmak 233:233–236

    Google Scholar 

  • Vlk J (1958b) Über den Acetylcholingehalt im rechten und linken Herzvorhof bei Kaninchen und Ratten. Naunyn-Schmiedebergs Arch Exp Path Pharmak 235:19–22

    Google Scholar 

  • Vlk J, Tuček S (1961) Distribution of acetylcholine in the auricles of the mammalian heart. Physiol Bohemoslov 10:65–71

    Google Scholar 

  • Vlk J, Tuček S (1964) The formation of acetylcholine in isolated heart auricles of white rats and guinea-pigs. Physiol Bohemoslov 13:310–314

    Google Scholar 

  • Vlk J, Tuček S (1965) Comparative study of acetylcholine metabolism in mammalian hearts. Fed Proc (Translation Suppl) 24:T821-T822

    Google Scholar 

  • Vlk J, Tuček S, Habermann V (1961) Distribution of acetylcholine and cholinesterase in the heart of white rats. Nature 189:923–924

    Google Scholar 

  • White HL, Wu JC (1973) Choline and carnitine acetyltransferases of heart. Biochemistry 12:841–846

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Physiology, Charles University Medical Faculty, CS-30605, Plzeň, Czechoslovakia

    Jana Slavíková

  2. Institute of Physiology, Czechoslovak Academy of Sciences, CS-14220, Prague, Czechoslovakia

    Stanislav Tuček

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  1. Jana Slavíková
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  2. Stanislav Tuček
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Slavíková, J., Tuček, S. Choline acetyltransferase in the heart of adult rats. Pflugers Arch. 392, 225–229 (1982). https://doi.org/10.1007/BF00584300

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  • DOI: https://doi.org/10.1007/BF00584300

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