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GTP-binding proteins mediate acetylcholine inhibition of voltage dependent calcium channels in hippocampal neurons

  • Excitable Tissues Cand Central Nervous Physiology
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Abstract

Acetylcholine (Ach) was tested for its effect on calcium currents in primary cultures of embryonic rat hippocampal neurones. 10 μM Ach reversibly depressed the high voltage activated (HVA) Ca current and the effect was blocked by atropine. When GTP binding proteins (G-proteins) were irreversibly activated by intracellularly perfusing the neurons with GTP[ S], one short-lasting application of Ach permanently inhibited Ca currents. Alternatively, the transmitter was inactive when the cells were preincubated with pertussis toxin, which inactivates some G-proteins by catalyzing ADP ribosylation. Our results suggest that a G-protein mediates the inhibitory modulation of the HVA Ca current by muscarinic agonists.

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Authors and Affiliations

  1. Abteilung Neurophysiologie, Max-Planck-Institut für Psychiatrie, Am Klopferspitz 18 A, D-8033, Planegg, Germany

    Mauro Toselli & Hans Dieter Lux

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  1. Mauro Toselli
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  2. Hans Dieter Lux
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Toselli, M., Lux, H.D. GTP-binding proteins mediate acetylcholine inhibition of voltage dependent calcium channels in hippocampal neurons. Pflugers Arch. 413, 319–321 (1989). https://doi.org/10.1007/BF00583548

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  • DOI: https://doi.org/10.1007/BF00583548

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