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Transforming growth factor ß1 acid interaction

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Abstract

Chick embryo skin fibroblasts release transforming growth factor β1 that is able to modulate glycosaminoglycan synthesis and secretion. When incubated with individual classes of glycosaminoglycans, the factor's modulatory activity was altered. To determine whether direct interactions between transforming growth factor β1 and glycosaminoglycans occur, we have assessed the activity of the growth factor after pre-incubation with single classes of glycosaminoglycans by assaying its inhibitory effect upon the proliferative response of thymocytes stimulated with interleukin-1. Untreated transforming growth factor β1 suppressed the proliferative response of thymocytes to interleukin-1, as did transforming growth factor β1 pre-incubated with sulphated glycosaminoglycans. By contrast, transforming growth factor β1 lost its inhibitory capacity when preincubated with high molecular weight hyaluronic acid. Digestion of transforming growth factor β1-hyaluronic acid complex with hyaluronidase released active transforming growth factor β1. Trypsin degraded transforming growth factor β1 alone, but did not degrade the transforming growth factor β1-hyaluronic acid complex. These results suggest that hyaluronic acid interacts with transforming growth factor β1, thus protecting the factor from tryptic degradation and may be a means of concentrating growth factor activity.

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Authors and Affiliations

  1. Dipartimento di Medicina Sperimentale e ScienzeBiochimiche, Università degli Studi di Perugia, I-06126, Perugia, Italia

    Paola Locci, Lorella Marinucci, Cinzia Lilli, Domenica Martinese & Ennio Becchetti

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  1. Paola Locci
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  2. Lorella Marinucci
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  3. Cinzia Lilli
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  4. Domenica Martinese
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  5. Ennio Becchetti
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Locci, P., Marinucci, L., Lilli, C. et al. Transforming growth factor ß1 acid interaction. Cell Tissue Res. 281, 317–324 (1995). https://doi.org/10.1007/BF00583400

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  • DOI: https://doi.org/10.1007/BF00583400

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