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The facilitating effect of gangliosides on the electrogenic (Na+/K+) pump and on the resistance of the membrane potential to hypoxia in neuromuscular preparation

  • Excitable Tissues and Central Nervous Physiology
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Abstract

The effects have been investigated of a mixture of gangliosides from beef brain cortex (GM1, GD1a, GD1b and GT1) either added to the bathing medium or injected intraperitoneally on muscle fibres and nerve terminals in mouse diaphragm. The electrogenic (Na+/K+) pump activity of muscle fibres enriched with sodium was increased by 38% after 2-h pretreatment with gangliosides (5×10−8 mol ·l−1). Muscles from animals treated with gangliosides did not show the substantial depolarization of the resting membrane potential (RMP) in K+-free solution (6 h) shown by control muscles. Further, treatment with gangliosides slowed the changes in muscle fibre RMP and frequency of the miniature end-plate potentials in oxygen deprived muscles.

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Author notes

  1. František Vyskočil

    Present address: Institute of Physiology, Czechoslovak Academy of Sciences, CS-142 20, Prague 4, Czechoslovakia

Authors and Affiliations

  1. Department of Cytopharmacology, FIDIA Research Laboratories, Abano Terme, Italy

    František Vyskočil, Franco Di Gregorio & Alfredo Gorio

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  1. František Vyskočil
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  2. Franco Di Gregorio
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  3. Alfredo Gorio
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Vyskočil, F., Di Gregorio, F. & Gorio, A. The facilitating effect of gangliosides on the electrogenic (Na+/K+) pump and on the resistance of the membrane potential to hypoxia in neuromuscular preparation. Pflugers Arch. 403, 1–6 (1985). https://doi.org/10.1007/BF00583273

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  • DOI: https://doi.org/10.1007/BF00583273

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