Abstract
Na−K-ATPase activity was determined in 10 segments of the rat nephron using a fluorometric microassay method [4]. The enzyme activity showed three peaks (>200 pmol ADP min−1 mm−1) along the nephron of normal rats. These peaks were in the S1 portion of the proximal tubule, the medullary thick ascending limb from the inner stripe and the distal convoluted tubule. Feeding the rats a low potassium diet for 8 weeks produced a significant decrease in Na−K-ATPase activity in the cortical collecting duct, but no significant change in this enzyme in any other segment. The low potassium diet did not produce a significant change in Mg-ATPase in any nephron segments. We conclude that Na−K-ATPase activity along the rat nephron shows a pattern that is qualitatively similar to that seen in the rabbit nephron [4]. However, quantitatively the Na−K-ATPase activity in the rat nephron is greater than in the corresponding segments of the rabbit nephron. The results are consistent with the greater rate of glomerular filtration and Na+ reabsorption per rat nephron. Furthermore, our results suggest that the decrease in potassium excretion during potassium deficiency is modulated, at leat in part, by the level of Na−K-ATPase activity in the cortical collecting duct.
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References
Allen F, Tisher CC (1976) Morphology of the ascending thick limb of Henle. Kidney Int 9:8–22
Doucet A, Katz AI (1980) Renal potassium adaptation: Na−K-ATPase activity along the nephron after chronic potassium loading. Am J Physiol 238:F380-F386
Fine LG, Yanagawa N, Schultze RG, Tuck M, Trizna W (1979) Functional profile of the isolated uremic nephron: Potassium adaptation in the rabbit cortical collecting tubule. J Clin Invest 64:1033–1043
Garg LC, Knepper M, Burg MB (1981) Mineralocorticoid effects on sodium and potassium adenosine triphosphatase in individual nephron segments. Am J Physiol 240:F536-F544
Giebisch G (1979) Renal potassium transport. In: Giebisch G, Tosteson DC, Ussing HH (eds) Membrane transport in biology. Vol 4B. Springer, Berlin Heidelberg New York, pp 215–298
Goldstein A (1964) Biostatistics: An introductory text. The Macmillan Co, New York
Mayes DM, Furuyama S, Kem DC, Nugent CA (1970) A radioimmunoassay for plasma aldosterone. J Clin Endocrinol Metab 30:682–685
Hansen GP, Tisher CC, Robinson RR (1980) Response of the collecting duct to disturbances of acid-base and potassium balance. Kidney Int 17:326–337
Jorgensen PL (1980) Sodium and potassium ion pump in kidney tubules. Physiol Rev 60:804–917
Katz AI, Doucet A, Morel F (1979) Na−K-ATPase activity along the rabbit, rat and mouse nephron. Am J Physiol 237:F114-F120
Linas LS, Peterson LN, Anderson RJ, Aisenbrey GA, Simon FR, Berl T (1979) Mechanism of renal potassium conservation in the rat. Kidney Int 15:601–611
O'Neil RG (1981) Potassium secretion by the cortical collecting tubule. Fed Proc 40:2403–2407
Rastegar A, Biemesderfer D, Kashgarian M, Hayslett JP (1980) Changes in membrane surfaces of collecting duct cells in potassium adaptation. Kidney Int 18:293–301
Rodriguez HJ, Hogan WC, Hellman RN, Klahr S (1980) Mechanism of activation of renal Na−K-ATPase in the rat: effect of potassium loading. Am J Physiol 238:F315-F323
Schmidt U, Horster M (1978) Sodium potassium activated adenosine triphosphatase. Methodology quantitation in microdissected renal tubule segments from freeze dried and fresh tissues. In: Martinez-Maldonado M (ed) Methods in pharmacology. Plenum, New York, pp 259–296
Silva P, Hayslett JP, Epstein FH (1973) The role of Na−K-adenosine triphosphatase in potassium adaptation. J Clin Invest 52:2665–2671
Silva P, Ross BD, Charney AN, Besarb A, Epstein FH (1975) Potassium transport by the isolated perfused kidney. J Clin Invest 56:862–869
Skou JC (1980) The Na−K-ATPase as a regular of the intracellular concentration of Na+, K+ and Cl−. Its function in transcellular transport of NaCl. In: Maunsbach AB, Olsen TS, Christensen EI (eds) Functional ultrastructure of the kidney. Academic Press, New York, p 165
Stanton BA, Biemesderfer D, Wade JB, Giebisch G (1981) Structural and functional study of the rat distal nephron: effects of potassium adaptation and depletion. Kidney Int 19:36–48
Stetson DL, Wade JB, Giebisch G (1980) Morphological alterations in the rat medullary collecting duct following potassium depletion. Kidney Int 17:45–56
Widhager EE (1979) Sodium chloride transport. In: Giebisch G, Tosteson DC, Ussing HH (eds) Membrane transport in biology, vol 4B. Springer, Berlin Heidelberg New York, pp 146–213
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Garg, L.C., Mackie, S. & Tisher, C.C. Effect of low potassium-diet on Na−K-ATPase in rat nephron segments. Pflugers Arch. 394, 113–117 (1982). https://doi.org/10.1007/BF00582911
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DOI: https://doi.org/10.1007/BF00582911