Abstract
The relationship between tissue oxygen partial pressure (pO2) values and electrical activities of guinea pig olfactory cortical slices was investigated as the slices were superfused with Krebs-Ringer's solution equilibrated with different gas mixtures. ThepO2 values were measured in the slices with oxygen microelectrodes (tip diameter <1 μm). 1. Studies ofpO2 measurements showed the variability of minimumpO2 value of oxygen profiles in the tissue slice. The profile depends on thepO2 value of the superfusate and on the thickness and the oxygen consumption of the slice. With our experimental conditions an anoxic area developed in the middle layers of the slice when the thickness of the slice exceeded ca. 430 μm; in thinner slices there was no anoxic area. In our case the limiting section thickness of the slice was ca. 430 μm from the viewpoint of tissuepO2 value. 2. The N potential (extra-cellularly recorded EPSP) showed a tendency to decrease in amplitude, for slices being thicker than ca. 430 μm. It would seem reasonable to think that the decrement of the N potential was brought about by the existence of the anoxic area. 3. When the slice was bubbled with 25, 45 or 95% O2, the tissuepO2 value changed, and the N potential height also changed. The N potential was higher in amplitude when bubbled with 95% O2 than with 25% O2. On the other hand, the amplitude of the IS potential (the lateral olfactory tract potential) was not influenced as much as that of the N potential by the change of tissuepO2 value in the slice. 4. The tissuepO2 value was continuously measured during the electrical stimulation of the lateral olfactory tract. The steady state level of tissuepO2 value obtained during the stimulation diminished as the frequency of stimulation increased from 5–30 Hz.
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Fujii, T., Baumgärtl, H. & Lübbers, D.W. Limiting section thickness of guinea pig olfactory cortical slices studied from tissuepO2 values and electrical activities. Pflugers Arch. 393, 83–87 (1982). https://doi.org/10.1007/BF00582396
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DOI: https://doi.org/10.1007/BF00582396