Summary
Leg and wing imaginal discs of mature larvae ofDrosophila melanogaster when treated with 0.1% trypsin for 5–10 min underwent a change in shape that closely resembled normal pupal morphogenesis. Simultaneously, the cells of the disc epithelium changed in shape from tall columnar to cuboidal. Colcemid eliminated microtubules but was without effect on the shape of the imaginal discs or their cells. Tryptic digestion reduced non-junctional intercellular adhesivity but septate desmosomes and gap junctions remained intact.
It is proposed that the structure of imaginal discs permits the packaging of the anlagen of the adult integument so that they can change shape and replace the larval structures in a brief period. Apparently most of the definitive form of the pupal leg is built into the disc and becomes visible within a few minutes as intercellular adhesivity is changed.
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References
Baker, C., Schroeder, T. E.: Cytoplasmic filaments and morphogenetic movement in the amphibian neutral tube. Develop. Biol.15, 432–450 (1967).
Bernfield, M. R., Wessels, N. K.: Intra- and extra-cellular control of epithelial morphogenesis. From a paper presented at the 29th Symposium of the Society for Developmental Biology, June 1970.
Bikle, D., Tilney, L. G., Porter, K. R.: Microtubules and pigment migration in the metamorphoses ofFundulus heteroclitus. Protoplasma (Wien)61, 322–345 (1966).
Bodenstein, D.: The postembryonic development ofDrosophila. In: Biology ofDrosophila, ed. M. Demerec. p. 267–367. New York: Hafner 1950.
Brinkley, B. R., Stubblefield, E., Hsu, T. C.: The effects of colcemid inhibition and reversal on the fine structure of the mitotic apparatus of chinese hamster cellsin vitro. J. Ultrastruct. Res.19, 1–18 (1967).
Bryant, P. J.: Cell lineage relationships in the imaginal wing disc ofDrosophila melanogaster. Develop. Biol.22, 389–411 (1970).
—, Schneiderman, H. A.: Cell lineage, growth and determination in the imaginal leg discs ofDrosophila melanogaster. Develop. Biol.20, 263–290 (1969).
DuPraw, E. J.: The organization of honey bee embryonic cells. I. Microtubules and ameboid activity. Develop. Biol.12, 53–71 (1965).
Freed, J. J., Lebowitz, M.: The association of a class of saltatory movements with microtubules in cultured cells. J. Cell Biol.45, 334–354 (1970).
Fristrom, D. K.: Ultrastructure of wild type and mutant strains ofDrosophila melanogaster. Ph. D. Thesis, University of California, Berkeley, 1969.
Fristrom, J. W., Raikow, R., Petri, W., Stewart, D.:In vitro evagination and RNA synthesis in imaginal discs ofDrosophila melanogaster. In: Problems in biology: RNA in development, ed. E. W. Hanley, p. 381–401. Salt Lake City: University of Utah Press 1970.
Garcia-Bellido, A.: Pattern reconstruction by dissociated imaginal disc cells ofDrosophila melanogaster. Develop. Biol.14, 278–306 (1966).
Gustafson, T., Wolpert, L.: The cellular basis of morphogenesis and sea urchin development. Int. Rev. Cytol.15, 139–214 (1963).
Hadorn, E., Buck, D.: Über Entwicklungsleistungen transplantierter Teilstücke von Flügel-Imaginalscheiben vonDrosophila melanogaster. Rev. suisse Zool.69, 302–310 (1962).
Hörstadius, S.: Über die animalisierende Wirkung von Trypsin auf Seeigelkeime. Zool. Jb. Abt. allg. Zool. u. Physiol.71, 241–244 (1965).
Karnovsky, M. J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol.27, 137 A (1965).
Markman, B., Runnström, J.: The removal by actinomycin D of the effects of endogenous or exogenous animalizing agents in sea urchin development. Wilhelm Roux' Archiv165, 1–7 (1970).
Perry, M. M.: Further studies on the development of the eye ofDrosophila melanogaster. I. The ommatidia. J. Morph.124, 227–248 (1968).
—: Further studies on the development of the eye ofDrosophila melanogaster. II. The interommatidial bristles. J. Morph.124, 249–262 (1968).
Poodry, C. A., Schneiderman, H. A.: The ultrastructure of the developing leg ofDrosophila melanogaster. Wilhelm Roux' Archiv166, 1–44 (1970).
Postlethwait, J. H., Schneiderman, H. A.: A clonal analysis of development inDrosophila melanogaster. Morphogenesis, determination and growth in the wild-type antenna. Develop. Biol.24, 477–519 (1971).
Runnström, J., Immers, J.: The animalizing action of trypsin on embryos of the sea urchin (Psammechinus milaris, Paracentrotus lividus). A study of interactions in early differentiation. Arch. Biol. (Liège)77, 365–410 (1966).
Schubiger, G.: Anlageplan, Determinationszustand und Transdeterminationsleistungen der männlichen Vorderbeinscheibe vonDrosophila melanogaster. Wilhelm Roux' Archiv160, 9–40 (1968).
Sprey, Th. E.: Morphological and histochemical changes during the development of some of the imaginal discs ofCalliphora erythrocephala. Neth. J. Zool.20, 253–275 (1970).
Stubblefield, E., Brinkley, B. R.: Cilia formation in chinese hamster fibroblastsin vitro as a response to colcemid treatment. J. Cell Biol.30, 645–652 (1966).
Tilney, L. G.: The assembly of microtubules and their role in the development of cell form. In: The emergence of order in developing systems, ed. M. Locke. New York: Academic Press 1968.
Ursprung, H., Schabtach, E.: The fine structure of the maleDrosophila genital disc during late larval and early pupal development. Wilhelm Roux' Archiv160, 243–254 (1968).
Wehman, H. J.: Fine structure ofDrosophila wing imaginal discs during early stages of metamorphosis. Wilhelm Roux' Archiv163, 375–390 (1969).
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Poodry, C.A., Schneiderman, H.A. Intercellular adhesivity and pupal morphogenesis inDrosophila melanogaster . W. Roux' Archiv f. Entwicklungsmechanik 168, 1–9 (1971). https://doi.org/10.1007/BF00582000
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DOI: https://doi.org/10.1007/BF00582000