Abstract
Intracellular element concentrations were measured in rat sympathetic neurones using energy dispersive electron microprobe analysis. The resting intracellular concentrations of sodium potassium and chloride measured in ganglia maintained for about 90 min in vitro at 25° C were 3, 155 and 25 mmol/kg total tissue wet weight respectively. Recalculated in mmol/l cell water, these values are 5, 196 and 32 respectively. There were no significant differences between the nuclear and cytoplasmic values of these ions. Incubation in either carbachol (108 μmol/l, 4 min) or ouabain (1 mmol/l, 60 min) significantly increased the intracellular sodium and decreased the intracellular potassium concentrations. Neither substance materially altered the intracellular chloride concentration. The data obtained are compared and contrasted to those obtained in mammalian sympathetic neurones using chemical analysis and ion-sensitive microelectrodes.
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References
Adams PR, Brown DA (1975) Actions of γ-aminobutyric acid on sympathetic ganglion cells. J Physiol (Lond) 250:85–120
Ballanyi K, Grafe P, ten Bruggencate G (1983) Intracellular free sodium and potassium, post-carbachol hyperpolarization, and extracellular potassium-undershoot in rat sympathetic neurones. Neurosci Lett 38:275–279
Bauer R, Rick R (1978) Computer analysis of x-ray spectra (EDS) from thin biological specimens. X-Ray Spectrom 7:63–69
Beck F, Bauer R, Bauer U, Mason J, Dörge A, Rick R, Thurau K (1980) Electron microprobe analysis of intracellular elements in the rat kidney. Kidney Int 17:756–763
Beck F, Dörge A, Mason J, Rick R, Thurau K (1982) Element concentrations of renal and hepatic cells under potassium depletion. Kidney Int 22:250–256
Brinley FJ (1967) Potassium accumulation and transport in the rat sympathetic ganglion. J Neurophysiol 30:1531–1560
Brown DA, Scholfield CN (1974a) Changes of intracellular sodium and potassium ion concentrations in isolated rat superior cervical ganglia induced by depolarizing agents. J Physiol (Lond) 242:307–319
Brown DA, Scholfield CN (1974b) Movements of labelled sodium ions in isolated rat superior cervical ganglia. J Physiol (Lond) 242:321–351
Constanti A, Brown DA (1981) M-currents in voltage-clamped mammalian sympathetic neurones. Neurosci Lett 24:289–294
Deitmer JW, Ellis D (1978) The intracellular sodium activity of cardiac purkinje fibres during inhibition and re-activation of the Na-K pump. J Physiol (Lond) 284:241–259
Dörge A, Rick R, Gehring K, Thurau K (1978) Preparation of freeze-dried cryosections for quantitative X-ray microanalysis of electrolytes in biological soft tissues. Pflügers Arch 373: 85–97
Förstl J, Galvan M, ten Bruggencate G (1982) Extracellular K+ concentration during electrical stimulation of rat isolated sympathetic ganglia, vagus and optic nerves. Neuroscience 7: 3221–3229
Grafe P, Rimpel J, Reddy MM, ten Bruggencate G (1982) Changes of intracellular sodium and potassium ion concentrations in frog spinal motoneurons induced by repetitive synaptic stimulation. Neuroscience 7:3212–3220
Harris EJ, McLennan H (1953) Cation exchanges in sympathetic ganglia. J Physiol (Lond) 121:629–637
Heinemann U, Lux HD, Gutnick MJ (1977) Extracellular free calcium and potassium during paroxysmal activity in the cerebral cortex of the cat. Exp Brain Res 27:237–243
Kriz N, Syková E, Ujec E, Vyklický L (1974) Changes of extracellular potassium concentration induced by neuronal activity in the spinal cord of the cat. J Physiol (Lond) 238:1–15
Krnjević K, Morris ME (1975) Factors determining the decay of K+ potentials and focal potentials in the central nervous system. Can J Physiol Pharmacol 53:923–934
Larrabee MG, Klingman JO (1962) Metabolism of glucose and oxygen in mammalian sympathetic ganglia at rest and in action. In: Page KAC, Page IH, Quastel JH (eds) Neurochemistry. Charles C. Thomas. Illinois, pp 150–176
Lux HD (1974) Fast recording ion specific microelectrodes: their use in pharmacological studies in the CNS. Neuropharmacology 13:509–517
Nagel W, Garcia-Diaz JF, Armstrong W McD (1981) Intracellular ionic activities in frog skin. J Membr Biol 61:127–134
Nicholson C, ten Bruggencate G, Stöckle H, Steinberg R (1978) Calcium and potassium changes in extracellular microenvironment of cat cerebellar cortex. J Neurophysiol 41:1026–1039
Palmer LG, Civan MM (1977) Distribution of Na+, K+, and Cl− between nucleus and cytoplasm inchironomus salivary gland cells. J Membr Biol 33:41–61
Rick R, Dörge A, Katz U, Bauer R, Thurau K (1980) The osmotic behaviour of toad skin epithelium (Bufo viridis). An electron microprobe analysis. Pflügers Arch 385:1–10
Rick R, Dörge A, MacNight ADC, Leaf A, Thurau K (1978a) Electron microprobe analysis of the different epithelial cells of toad urinary bladder. J Membr Biol 39:257–271
Rick R, Dörge A, Thurau K (1982) Quantitative analysis of electrolytes in frozen dried sections. J Microsc 125:239–247
Rick R, Dörge A, Tippe A (1976) Elemental distribution of Na, P, Cl and K in different structures of myelinated nerve ofRana esculata. Experientia 32:1018–1019
Rick R, Dörge A, von Arnim E, Thurau K (1978b) Electron microprobe analysis of frog skin epithelium: Evidence for a syncytial sodium transport compartment. J Membr Biol 39:313–331
Sansone FM, McIsaac RJ, Tomasulo JA (1982) Morphological integrity of the rat superior cervical ganglion after prolonged incubation in vitro. Acta Anat 112:137–141
Scholfield CN (1977) Movements of radioactive potassium in isolated rat ganglia. J Physiol (Lond) 268:123–137
Vogt M (1936) Potassium changes in stimulated superior cervical ganglion. J Physiol (Lond) 86:258–263
Woodward JK, Bianchi CP, Erulkar SD (1969) Electrolyte distribution in rabbit superior cervical ganglion. J Neurochem 16: 289–299
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Galvan, M., Dörge, A., Beck, F. et al. Intracellular electrolyte concentrations in rat sympathetic neurones measured with an electron microprobe. Pflugers Arch. 400, 274–279 (1984). https://doi.org/10.1007/BF00581559
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DOI: https://doi.org/10.1007/BF00581559