Abstract
Microelectrodes were used to record the effects of glucose on the membrane potential of single mouse B cells. In most cells, the slow waves of depolarization and the intervals of repolarization produced by a constant concentration of glucose displayed a great regularity. However, cyclic variations in the duration of these slow waves and/or intervals were observed in a certain number of B cells. These oscillations were more clearly visible and more frequent (47%) in the presence of 15 mM glucose, than in the presence of 10 mM glucose (19%). They sometimes disappeared with time, but sometimes persisted for over 90 min and were not affected by atropine, propanolol and phentolamine. Their mean period was 203 s at 10 mM glucose and 235 s at 15 mM glucose. The membrane potential and the degree of electrical activity were not different in B cells exhibiting these cyclic variations or not. These oscillations in the duration of slow waves and intervals induced by glucose could be due to fluctuations in metabolic events and in cytoplasmic K+ activity in B cells.
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Atwater I, Dawson CM, Ribalet B, Rojas E (1979) Potassium permeability activated by intracellular calcium ion concentration in the pancreatic B-cell. J Physiol (Lond) 288:575–588
Atwater I, Dawson CM, Scott A, Eddlestone G, Rojas E (1980) The nature of the oscillatory behaviour in electrical activity from pancreatic B-cell. Horm Metab Res [Suppl] 10:100–107
Baetens D, Stefan Y, Ravazzola M, Malaisse-Lagae F, Coleman DL, Orci L (1978) Alteration of islet cell populations in spontaneously diabetic mice. Diabetes 27:1–7
Beigelman PM, Thomas LJ, Slavin B, Shu MJ, Bessman SP (1973) Insulin from individual isolated islets of Langerhans. Response to glucose. Biochem Med 8:392–402
Dean PM, Matthews EK (1970) Glucose-induced electrical activity in pancreatic islet cells. J Physiol (Lond) 210:255–264
Dean PM, Matthews EK, Sakamoto Y (1975) Pancreatic islet cells: effects of monosaccharides, glycolytic intermediates and metabolic inhibitors on membrane potential and electrical activity. J Physiol (Lond) 246:459–478
Garcia-Hermida O, Gomez-Acebo J (1974) A different pattern of insulin release in the perfused islets of the rabbit. Biochem Biophys Res Commun 57:209–215
Henquin JC (1979) Opposite effects of intracellular calcium and glucose on the potassium permeability of pancreatic islet cells. Nature 280:66–68
Henquin JC (1980) Metabolic control of the potassium permeability in pancreatic islet cells. Biochem J 186:541–550
Henquin JC, Meissner HP (1981) Effects of amino acids on membrane potential and86Rb+ fluxes in pancreatic B cells. Am J Physiol 240:E245-E252
Malaisse WJ, Boschero AC, Kawazu S, Hutton JC (1978) The stimulus-secretion coupling of glucose-induced insulin release. Effect of glucose on K+ fluxes in isolated islets. Pflügers Arch 373:237–242
Malaisse WJ, Sener A, Herchuelz A, Valverde I, Hutton JC, Atwater I, Leclercq-Meyer V (1980) The interplay between metabolic and cationic events in islet cells: coupling factors and feedback mechanisms. Horm Metab Res (Suppl) 10:61–66
Matthews EK, O'Connor MDL (1979) Dynamic oscillations in the membrane potential of pancreatic islet cells. J Exp Biol 81:75–91
Meissner HP (1976) Electrical characteristics of the beta cells in pancreatic islets. J Physiol (Paris) 72:757–767
Meissner HP, Preissler M (1978) Influence of somatostatin on the membrane potential of B cells. Diabetologia 14:255
Meissner HP, Preissler M (1979) Glucose-induced changes in the membrane potential of pancreatic B cells: their significance for the regulation of insulin release. In: Camerini-Davalos RA, Hanover B (eds) Treatment of early diabetes. Plenum Press. New York, pp 97–107
Meissner HP, Preissler M, Henquin JC (1980) Possible ionic mechanisms of the electrical activity induced by glucose and tolbutamide in pancreatic B cells. In: Waldhäusl K (ed) Diabetes 1979. Excerpta Medica, Amsterdam, pp 166–171
Meissner HP, Schmelz H (1974) Membrane potential of B-cells in pancreatic islets. Pflügers Arch 351:195–206
Pace CS, Matschinsky FM, Lacy PE, Conant S (1977) Electrophysiological evidence for the autoregulation of B-cell secretion by insulin. Biochim Biophys Acta 497:408–414
Pace CS, Tarvin JT (1981) Somatostatin: mechanism of action in pancreatic islet B-cells. Diabetes 30:836–842
Ribalet B, Beigelman PM (1979) Cyclic variation of K+ conductance in pancreatic B-cells: Ca2+ and voltage dependence. Am J Physiol 237:C137-C146
Smith PH, Porte D (1976) Neuropharmacology of the pancreatic islets. Ann Rev Pharmacol Toxicol 16:269–285
Stagner JI, Samols E, Weir GC (1980) Sustained oscillations of insulin, glucagon and somatostatin from the isolated canine pancreas during exposure to a constant glucose concentration. J Clin Invest 65:939–942
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Henquin, J.C., Meissner, H.P. & Schmeer, W. Cyclic variations of glucose-induced electrical activity in pancreatic B cells. Pflugers Arch. 393, 322–327 (1982). https://doi.org/10.1007/BF00581418
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DOI: https://doi.org/10.1007/BF00581418