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Atrial natriuretic peptide receptors along the rat and rabbit nephrons: [125I] α-rat atrial natriuretic peptide binding in microdissected glomeruli and tubules

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Abstract

Binding of [125I] α-rat atrial natriuretic peptide ([125I] α-RANP) was measured in glomeruli and pieces of tubule microdissected from rat and rabbit nephrons. High densities of specific ANP binding sites were found only in the glomeruli (10–30×10−18 mol·glom−1), whereas no specific binding could be detected in the proximal tubule, the thin segments of the Henle's loop, the thick ascending limb, the distal tubule and the cortical and outer medullary collecting tubules. Rising the temperature from 4° C to 35° C resulted in biphasic kinetics of binding, suggesting a temperature-dependent inactivation of labelled hormone by glomeruli. At 4° C, specific binding of [125I] α-RANP was time and dose-dependent and Scatchard analysis of data indicated an apparent equilibrium dissociation constant of 0.63 nM. Competition experiments revealed the following sequence of stereospecificity for binding to rat glomeruli: RANP 3–28>[125I] α-RANP=[125I] α-HANP=α-RANP=atriopeptin III > atriopeptin II, whereas binding was unaffected by pharmacological doses of unrelated peptide hormones, prostaglandins, adrenergic agonists, dopamine, histamine and carbamylcholine. The results indicate that glomerular binding sites might be the physiological ANP receptors.

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Abbreviations

ANP:

atria natriuretic peptide

α0RANP:

alpha rat atrial natriuretic peptide

PCT:

proximal convoluted tubule

PR:

pars recta

TDL:

thin descending limb

TAL:

thin ascending limb

MAL:

medullary thick ascending limb

CAL:

dortical thick ascending limb

DCT:

distal convoluted tubule

DCTb:

distal convoluted tubule bright

DCTg:

distal convoluted tubule granular

CCT:

conrtical collecting tubule

MCT:

outer medullary collecting tubule

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Authors and Affiliations

  1. Laboratoire de Physiologie Cellulaire, Collège de France, 11, Place Marcelin Berthelot, F-75231, Paris Cedex 05, France

    Daniel Butlen, M'Hamed Mistaoui & François Morel

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  1. Daniel Butlen
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  2. M'Hamed Mistaoui
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  3. François Morel
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Butlen, D., Mistaoui, M. & Morel, F. Atrial natriuretic peptide receptors along the rat and rabbit nephrons: [125I] α-rat atrial natriuretic peptide binding in microdissected glomeruli and tubules. Pflugers Arch. 408, 356–365 (1987). https://doi.org/10.1007/BF00581129

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  • DOI: https://doi.org/10.1007/BF00581129

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