Abstract
The volume and surface densities of structural components in isolated long-sarcomere fibres of crayfish (Astacus fluviatilis) were estimated by stereological methods. The relative volumes were as follows (in % of the fibre volume): sarcolemmal invaginations (SI) 0.74; T-system 1.1; whole sarcoplasmic reticulum (SR) 4.7; junctional SR cisternae 0.41; mitochondria 1.1; nuclei 0.57; myofibrils 81.6; peripheral basal lamina 1.1; junctional gap 0.043; lysosomes 0.029. The values for surface densities were (in μm2/100 μm3): peripheral sarcolemma 1.27; SI 7.21; T-system 40.2; whole SR 146.4; junctional SR cisternae 14.88; junctional area of SR cisternae 7.05; mitochondria 8.98; nuclei 0.75; myofibrils 163. The invaginated membranes (SI and T-system) made up 97.4% of the total area of surface membranes and increased the effective area of peripheral sarcolemma 39 times. Using the values of the total electrical capacity from previous investigations onAstacus fibres, the specific capacity of surface membranes was calculated as 1.08 μF/cm2.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dulhunty AF, Franzini-Armstrong C (1975) The relative contribution of the folds and caveolae to the surface membrane of frog skeletal muscle fibres at different sarcomere lengths. J Physiol (Lond) 250:513–539
Eisenberg RS (1967) The equivalent circuit of single crab muscle fibers as determined by impedance measurements with intracellular electrodes. J Gen Physiol 50:1785–1806
Eisenberg B, Eisenberg RS (1968) Selective disruption of the sarcotubular system in frog sartorius muscle. A quantitative study with exogenous peroxidase as a marker J Cel Biol 39:451–467
Eisenberg BR, Kuda AM (1975) Stereological analysis of mammalian skeletal muscle. II. White Vastus muscle of the adult guinea pig. J Ultrastr Res 51:176–187
Eisenberg BR, Mobley BA (1975) Size changes in single muscle fibres during fixation and embedding. Tissue and Cell 7:383–387
Eisenberg BR, Kuda AM, Peter JB (1974) Stereological analysis of mammalian skeletal muscle. Part I. Soleus muscle of the adult guinea pig. J Cell Biol 60:732–754
Falk G, Fatt P (1964) Linear electrical properties of striated muscle fibres observed with intracellular electrodes. Proc Roy Soc B 160:69–123
Fatt P, Katz B (1953) The electrical properties of crustacean muscle fibres. J Physiol (Lond) 120:171–204
Henček M, Zachar J (1965) The electrical constants of single muscle fibres of the crayfish (Astacus fluviatilis) Physiol. Bohemoslov. 14:297–311
Karnovsky MJ (1967) The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J Cell Biol 35:213–236
Kilarski W (1973) Cytomorphometry of sarcoplasmic reticulum in extrinsic eye muscles of the teleost (Tinca tinca L.). Z Zellforsch 136:535–544
Mobley BA, Eisenberg BR (1975) Sizes of components in frog skeletal muscle measured by methods of stereology. J Gen Physiol 66:31–45
Novotová M, Uhrík B, Zachar J (1978) The extension of sarcolemmal invaginations in crayfish muscle fibres. Biológia (Bratislava) 33:693–700
Peachey LD (1967) Membrane systems of crab fibers. Am Zoologist 7:505–513
Poledna J, Zachar J, Zacharová D (1976) Square wave analysis of the Falk-Fatt model in isolated muscle fibres. Physiol Bohemoslov 25:569–572
Poledna J, Zachar J, Zacharová D (1976) Electrical properties of the surface and the T system membranes determined from the voltage transients. Biológia (Bratislava) 33:929–938
Rossner KL, Sherman RG (1978) Invaginated membrane in crustacean tonic muscle fibers: estimates of membrane capacitance. Am J Physiol 235 (5):C200-C226
Uhrík B (1975) Ultrastructural picture of the subsarcolemmal region of crayfish muscle fibre reconstructed from serial sections. Physiol Bohemoslov 24:91
Uhrík B, Zacharová D (1979) Three-dimensional arrangement of sarcoplasmic reticulum in crayfish as seen by high voltage electron microscope. Cell Tissue Res 202:343–346
Weibel ER (1972) A stereological method for estimating volume and surface of sarcoplasmic reticulum. J Microse 95:229–242
Weibel ER, Bolender RP (1973) Stereological techniques for electron microscopic morphometry. In: Hayat MA (ed) Principles and techniques of electron microscopy. Vol 3, Van Nostrand Reinhold Company, New York, pp 237–296
Zachar J (1971) Electrogenesis and contractility in skeletal muscle cells. University Park Press, Baltimore-London
Zacharova D, Uhrík B (1978) Kinetics of functional and morphological changes during decoupling and recoupling induced by glycerol in isolated muscle fibres of the crayfisch. Cell Tiss Res 192:167–185
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Uhrík, B., Novotová, M. & Zachar, J. A quantitative estimation of components in crayfish muscle fibres by stereological methods. Pflugers Arch. 387, 281–286 (1980). https://doi.org/10.1007/BF00580981
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00580981