Summary
The effect of renal ischemia of 15 s to 60 min duration on the tissue levels of adenosine, inosine and hypoxanthine was investigated in Sprague Dawley rats. A sharp increase in the tissue levels of adenosine from 5.13±0.56 to 31.3±2.96 nmol/g wet weight after 1 min of ischemia was found. The tissue levels of inosine and hypoxanthine in the controls were 3.62±0.51 and 3.19±0.76 nmol/g wet weight, respectively. Maximal levels of adenosine (38.1±6.3 nmol/g wet weight) were reached after 10 min of ischemia. The hypoxanthine levels rose steadily up to 922±183 nmol/g wet weight after 60 min of ischemia. Recirculation of 15 min after 60 min ischemia resulted in a fall of adenosine and inosine levels to values comparable to controls, whereas hypoxanthine was elevated above control values. In a second experimental series with tracing of renal blood flow (RBF) by a means of an electromagnetic flow meter a transient marked reduction of RBF after occlusion of the renal artery for 30 s was observed. The 3-fold increase of adenosine tissue levels within 30 s of renal artery occlusion and the inhibition of the postocclusive RBF reduction by theophylline (3.3 μmol/100 g body weight) make it likely that this phenomenon may be caused by intrarenal adenosine.
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Osswald, H., Schmitz, H.J. & Kemper, R. Tissue content of adenosine, inosine and hypoxanthine in the rat kidney after ischemia and postischemic recirculation. Pflugers Arch. 371, 45–49 (1977). https://doi.org/10.1007/BF00580771
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DOI: https://doi.org/10.1007/BF00580771