Skip to main content
Log in

On the false positive urease activity ofYarrowia lipolytica

  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

The ability ofYarrowia lipolytica to produce ammonia from urea was found variable on some media. The colour change of the indicator in Christensen's urea agar was not due to the urease activity of this species but was a non-specific alkalization reaction. Rapid urea broth was reliable giving no false positive results. It was found thatY. lipolytica is a urease negative yeast species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Barnett JA, Payne RW & Yarrow D (1983) Yeast: Characteristics and Identification. Cambridge Univ. Press, Cambridge

    Google Scholar 

  • , (1990) Yeasts: Characteristics and Identification, 2nd ed. Cambridge Univ. Press, Cambridge

    Google Scholar 

  • Booth JL & Vishniac HS (1987) Urease testing and yeast taxonomy. Can. J. Microbiol. 33: 396–404

    Google Scholar 

  • Christensen WB (1946) Urea decomposition as a means of differentiating Proteus and Paracolon cultures from each other and from Salmonella and Shigella types. J. Bacteriol. 52: 461–466

    Google Scholar 

  • hagler AN & Ahearn DG (1981) Rapid diazonium blue B test to detect basidiomycetous yeasts. Int. J. Syst. Bacteriol. 31: 204–208

    Google Scholar 

  • Kocková-Kratochvilová A (1982) Urease and extracellular nucleases of yeast. Folia Microbiol. 27: 404–412

    Google Scholar 

  • Kreger-van Rij NJW (Ed) (1984) The Yeasts, a Taxonomic Study, 3rd ed. Elsevier Science Publ., Amsterdam

    Google Scholar 

  • Moore RT (1980) Taxonomic proposals for the classification of marine yeasts and other yeast-like fungi including the smuts. Bot. Marina 23: 361–373.

    Google Scholar 

  • Roberts GD, Horstmeier CD, Land GA & Foxworth JH (1978) Rapid urea broth test for yeasts. J. Clin. Microbiol. 7: 584–588

    Google Scholar 

  • Seeliger HRP (1956) Use of a urease test for the screening and identification of cryptococci. J. Bacteriol. 72: 127–131

    Google Scholar 

  • Stuart CA, vanStratum E & Rustigian R (1945) Further studies on urease production by Proteus and related organisms. J. Bacteriol. 49: 437–444

    Google Scholar 

  • Weijman ACM, Rodrigues de Miranda L & van derWalt JP (1988) Redefinition of Candida Berkhout and the consequent emendation of Cryptococcus Kützing and Rhodotorula Harrison. Antonie van Leeuwenhoek 54: 545–553

    Google Scholar 

  • Wickerham LJ (1946) A critical evaluation of the nitrogen assimilation tests commonly used in the classification of yeasts. J. Bacteriol. 52: 293–301

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Péter, G., Deák, T. On the false positive urease activity ofYarrowia lipolytica . Antonie van Leeuwenhoek 60, 55–59 (1991). https://doi.org/10.1007/BF00580442

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00580442

Key words

Navigation