Summary
The regulation of the synthesis of alcohol oxidase, catalase, formaldehyde dehydrogenase, and formate dehydrogenase was investigated in the methanol-utilizing yeastHansenula polymorpha during growth on different carbon and energy sources. When cells were grown on glucose, the enzymes of the dissimilatory methanol metabolism were not detected during the exponential phase of growth, but were formed in the late stationary phase without addition of methanol. Moreover, the enzymes were synthesized during growth on sorbitol, glycerol, ribose, and xylose. It was shown that the carbon catabolite insensitivity of the synthesis of methanol-specific enzymes is not limited to substrates that are slowly metabolized.
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Bensadoun, A., Weinstein, D. (1976). Anal. Biochem.70, 241–250
Van Dijken, J.P., Veenhuis, M., Vermeulen, C.A., Harder, W. (1975a). Arch. Microbiol.105, 261–267
Van Dijken, J.P., Veenhuis, M., Kreger-van Rij, N.J.W., Harder, W. (1975b). Arch. Microbiol.102, 41–44
Van Dijken, J.P., Otto, R., Harder, W. (1976). Arch. Microbiol.111, 137–144
Fukui, S., Tanaka, A., Kawamoto, S., Yasuhara, S., Teranishi, S., Osumi, M. (1975). J. Bacteriol.123, 317–328
Kato, N., Tani, Y., Ogata, K. (1973). Agr. Biol. Chem.38, 675–677
Kesler, R.B., (1967). Analyt. Chem.39, 1416–1420
Leupold, U. (1970). Methods in Cell. Physiol.4, 169–177
Lowry, O.H., Rosebrough, N.J., Farr, J.G., Randall, R.J.(1951). J. Biol. Chem.193, 265–275
Metzenberg, R.L. (1972). Ann. Rev. Genetics6, 111–132
Polakis, E.S., Bartley, W. (1965). Biochem. J.97, 284–297
Roggenkamp, R., Sahm, H., Wagner, F. (1974). FEBS Lett.41, 283–286
Roggenkamp, R., Sahm, H., Hinkelmann, W., Wagner, F. (1975). Eur. J. Biochem.59, 231–236
Sahm, H., Wagner, F. (1973a). Arch. Microbiol.90, 263–268
Sahm, H., Wagner, F. (1973b). Eur. J. Biochem.36, 250–256
Sahm, H., Roggenkamp, R., Hinkelmann, W., Wagner, F. (1975). J. Gen. Microbiol.88, 218–222
Sahm, H. (1977). Adv. Biochem. Eng.6, 77–103
Schlanderer, G., Dellweg, H. (1974). Eur. J. Biochem.49, 305–316
Schütte, H., Flossdorf, J., Sahm, H., Kula, M.-R. (1976). Eur. J. Biochem.62, 151–160
Stanier, R.Y., Duodoroff, M., Adelberg, E.A. (1976). General Microbiology. London and Basingstoke: Macmillan Student Editions
Tani, Y., Miya, T., Nishikawa, H., Ogata, K. (1972). Agr. Biol. Chem.36, 68–74
Veenhuis, M., van Dijken, J.P., Harder, W. (1976). Arch. Microbiol.111, 123–135
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Eggeling, L., Sahm, H. Derepression and partial insensitivity to carbon catabolite repression of the methanol dissimilating enzymes inHansenula polymorpha . European J. Appl. Microbiol. Biotechnol. 5, 197–202 (1978). https://doi.org/10.1007/BF00579339
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DOI: https://doi.org/10.1007/BF00579339