Summary
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1.
Protein composition of rat brain, and of heart and skeletal muscle was analysed by polyacrylamide gel electrophoresis in their late fetal and postnatal development.
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2.
Adult organs show specificity of their protein patterns as judged from the relative quantitative proportions and distribution of separated protein bands.
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3.
Protein composition of morphologically already well defined organs in the late fetal stage of development is not changed by the event of birth and is preserved unmodified until the early neonatal period.
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4.
Significant developmental changes in protein composition leading to formation of adult pattern take place between the 3rd and 30th postnatal day.
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5.
Results and limitations of the technique applied are evaluated from the point of applicability to developmental studies.
Zusammenfassung
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1.
Das Protein-Muster von Gehirn, Herz und Skelettmuskel in spätembryonalen und postnatalen Entwicklungsstadien wurde durch Polyacrylamid-Gel-Elektrophorese untersucht.
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2.
Adultorgane zeigen Spezifität ihrer Eiweißmuster mit Bezug auf relative Anteile und Verteilung der getrennten Eiweißbanden.
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3.
Die Eiweiß-Zusammensetzung von morphologisch bereits gut definierten Organen im spätembryonalen Stadium wird nicht verändert durch den Vorgang der Geburt und bleibt erhalten bis in die frühe neonatale Periode.
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4.
Signifikante Entwicklungsänderungen in der Eiweiß-Zusammensetzung, die zur Bildung des adulten Musters führen, finden zwischen dem 3. und 30. postnatalen Tag statt.
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5.
Die Ergebnisse und die Limitationen der Methode werden beurteilt mit Bezug auf Anwendbarkeit auf entwicklungsbiologische Studien.
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With technical assistance of Maryann Eliason.
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Šerman, D., Škreb, N. Protein patterns during final differentiation of some rat organs. W. Roux' Archiv f. Entwicklungsmechanik 165, 277–284 (1970). https://doi.org/10.1007/BF00573674
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DOI: https://doi.org/10.1007/BF00573674