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Amperometrisches Verfahren zur Bestimmung der Aktivität von Cholinesterasen und Nachweis der Inhibitoren dieser Enzyme

Amperometric method for the determination of the activity of cholinesterases and detection of the inhibitors of the enzymes

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Summary

An electrochemical procedure based on the anodic oxidation of thiocholine iodide for the determination of the activity of cholinesterase both in serum and in the red blood cells is described. Using electronic differentiation of the current time curve the enzyme activity is directly available. The values obtained agree very well with the established method. Furthermore, both reversible and irreversible inhibitors of the cholinesterase have been quantified by using standardized serum samples.

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Authors and Affiliations

  1. Abt. Labor, Einheit Kreiskrankenhaus/Kreispoliklinik Burg, DDR-3270, Burg, Deutsche Demokratische Republik

    R. Gruß

  2. Zentralinstitut für Molekularbiologie, Bereich Enzymologie, Akademie der Wissenschaften der DDR, DDR-1115, Berlin-Buch, Deutsche Demokratische Republik

    F. Scheller

Authors
  1. R. Gruß
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  2. F. Scheller
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Gruß, R., Scheller, F. Amperometrisches Verfahren zur Bestimmung der Aktivität von Cholinesterasen und Nachweis der Inhibitoren dieser Enzyme. Z. Anal. Chem. 333, 29–32 (1989). https://doi.org/10.1007/BF00572613

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  • DOI: https://doi.org/10.1007/BF00572613

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