Skip to main content
SpringerLink
Log in

199mHg-derivatives of ascorbate oxidase and laccase: selective depletion and blocking of Cu-sites

  • Section IV: Application To Interdisciplinary Areas
  • Published:
Hyperfine Interactions Aims and scope Submit manuscript

Abstract

We report on the199mHg nuclear quadrupole interaction (NQI) of Hg-derivatives of the blue oxidases ascorbate oxidase (AO) and laccase (LAC). For fully reconstituted enzymes, three different NQIs were observed. The assignment of these NQIs to the type-1, -2, and -3 Cusites is based on type-2 depleted AO, on blocking studies with inactive Hg prior to199mHg/carrier reconstitution, and on the population ratio observed for fully reconstituted LAC. The NQIs for both enzymes are similar, suggesting similar Cu-sites. The type-2 site is preferentially reconstituted, contrary to expectations. Neither the blocking nor the depletion is as selective as expected.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. A. Messerschmidt, A. Rossi, R. Ladenstein, R. Huber, M. Bolognesi, G. Gatti, A. Marchesini, R. Petruzelli and A. Finazzi-Agró, J. Mol. Biol. 206 (1989) 513.

    Article  Google Scholar 

  2. J.L. Cole, G.O. Tan, E.K. Yang, K.O. Hodgson and E.I. Solomon, J. Am. Chem. Soc. 112 (1990) 2243.

    Article  Google Scholar 

  3. B. Reinhammar and Y. Oda, J. Inorg. Biochem. 11 (1979) 115.

    Article  Google Scholar 

  4. R. Malkin, B.G. Malmström and T. Vännegård, Eur. J. Biochem. 7 (1969) 253.

    Article  Google Scholar 

  5. M.T. Graziani, L. Morpurgo, G. Rotilio and B. Mondoví, FEBS Lett. 70 (1976) 87.

    Article  Google Scholar 

  6. M.M. Morie-Bebel, M.C. Morris, J.L. Menzie and D.R. McMillin, J. Am. Chem. Soc. 106 (1984) 3677.

    Article  Google Scholar 

  7. T. Butz, S. Saibene, Th. Fraenzke and M. Weber, Nucl. Instr. Meth. A 284 (1989) 417.

    Article  ADS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Physik-Department, Technische Universität München, 85747, Garching, Germany

    T. Butz & W. Tröger

  2. Max-Planck-Institut für Biochemie, 82152, Martinsried, Germany

    A. Messerschmidt, U. Thoenes & R. Huber

Authors
  1. T. Butz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Tröger
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Messerschmidt
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. U. Thoenes
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Huber
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Butz, T., Tröger, W., Messerschmidt, A. et al. 199mHg-derivatives of ascorbate oxidase and laccase: selective depletion and blocking of Cu-sites. Hyperfine Interact 80, 1127–1132 (1993). https://doi.org/10.1007/BF00567475

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00567475

Keywords

Search

Navigation