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Characterization of the synthesized collagen by mouse L-929 (L-5) cells

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Summary

In 48h incubation L-929 (L-5) cells secreted collagenous protein accounting for 16% of the total protein. CM-chromatography, SDS-acrylamide gel electrophoresis and DEAE-cellulose chromatography revealed that the collagenous protein was mainly composed of type I collagen, having no collagen of type III.

In addition, precursor-form collagen and tropocollagen molecules were found to be present in the incubate.

Zusammenfassung

L-929 (L-5) Mausfibroblasten synthetisieren bei einer 48-stündigen in vitro Inkubation kollagene Proteine mit einem Anteil von 16% am Gesamtprotein. Mit CM-Chromatographie, SDS-acrylamidgelelektrophorese und DEAE-Chromatographie wurde gezeigt, daß praktisch ausschließlich Typ I Kollagen synthetisiert wird, während Typ III Kollagen nicht nachweisbar ist. Zusätzlich fanden sich Kollagenvorläufer und Tropokollagen im Inkubationsmedium.

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Authors and Affiliations

  1. Department of Dermatology, School of Medicine, Osaka University, Fukushima, Fukushima-ku, 553, Osaka, Japan

    Shuho Matsubayashi, Hiroshi Shinkai & Shigeharu Sano

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  1. Shuho Matsubayashi
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  2. Hiroshi Shinkai
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  3. Shigeharu Sano
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Offprint requests to: Hiroshi Shinkai, M. D. (address see above)

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Matsubayashi, S., Shinkai, H. & Sano, S. Characterization of the synthesized collagen by mouse L-929 (L-5) cells. Arch. Derm. Res. 260, 7–15 (1977). https://doi.org/10.1007/BF00558009

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  • DOI: https://doi.org/10.1007/BF00558009

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