Abstract
The restriction cleavage sites of theBamHI-B andBamHI-E DNA fragments of several Herpes simplex virus type 1 (HVS-1) strains were mapped. These fragments are situated at the ends of the long unique regions and share homologous sequences in the repeat components (TRL and IRL) of the genome. All the strains analyzed were found to have deletions in the Hpal-P fragment, situated in theBamHI-B fragment. Five strains were further analyzed and the deletions were located in the Smal-A fragment (within the Hpal-P fragment). TheBamHI-E fragment of four recombinants (obtained by recombination between the HFEM genome and theBamHI-B fragment or part of it from the HSV-1 F strain) were almost identical but differed from another strain [NIH(LP)]. Comparison of theBamHI-B and theBamHI-E fragments of the same strain revealed that the fragments were not identical in all cases.
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Moyal, M., Raibstein, I., Rösen, A. et al. Heterogeneity ofBamHi DNA fragments B and E in several HSV-1 strains and recombinants. Virus Genes 1, 165–174 (1988). https://doi.org/10.1007/BF00555935
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DOI: https://doi.org/10.1007/BF00555935