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Betaine aldehyde dehydrogenase polymorphism in spinach: Genetic and biochemical characterization

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Abstract

Spinach (Spinacia oleracea L.) has a major chloroplastic isozyme of betaine aldehyde dehydrogenase (BADH) and a minor cytosolic one. Among a diverse collection of spinach accessions, three electrophoretic banding patterns of chloroplastic BADH were found: two were single banded and one was triple banded. Genetic analysis of these patterns indicated that chloroplastic BADH is encoded by a single, nuclear gene with two alleles, designated slow (S) and fast (F), and that products of these alleles can hybridize to form either homodimers or a heterodimer. The S allele was by far the most common among the accessions examined. Native and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the SS and FF homodimers differ in charge but not molecular weight.

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Authors and Affiliations

  1. MSU-DOE Plant Research Laboratory, Michigan State University, 48824, East Lansing, Michigan

    Elizabeth A. Weretilnyk & Andrew D. Hanson

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  1. Elizabeth A. Weretilnyk
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  2. Andrew D. Hanson
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This research was supported by the U.S. DOE under Contract DE-AC02-76ERO-1338, by USDA-CRGO Grant 87-CRCR-1-2460, and by grants from the State of Michigan REED-Biotechnology Program, from CIBA-GEIGY Corporation, and from the Beet Sugar Development Foundation.

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Weretilnyk, E.A., Hanson, A.D. Betaine aldehyde dehydrogenase polymorphism in spinach: Genetic and biochemical characterization. Biochem Genet 26, 143–151 (1988). https://doi.org/10.1007/BF00555495

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  • DOI: https://doi.org/10.1007/BF00555495

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