Abstract
Polyacrylamide gel electrophoresis and a kinetic microplate assay were used to detect heterozygotes resulting from a cross between B-type and non B-type strains of the whiteflyBemisia tabaci. Both strains were homozygous for different esterase and acetylcholinesterase (AChE) enzymes, and heterozygotes were produced in one of two crosses between B-type females (diploid) and non B-type males (haploid). In reciprocal crosses, however, no female offspring were produced, indicating that fertilization had not occurred. Despite the identification of individuals heterozygous for the esterase and AChE markers, there was clearly a significant degree of reproductive incompatibility between the two strains. The biochemical assays provided a vital component of this study and the advantages of their use are discussed.
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References
Bartlett, A. C., and Gawel, N. J. (1993). Determining whitefly species.Science (Technical comments 2611333.
Bedford, I. D., Briddon, R. W., Markham, P. G., Brown, J. K., and Rosell, R. C. (1993). A new species ofBemisia or biotype ofBemisia tabaci (Genn.) as a future pest of European agriculture.Plant Health and the European Single Market, BCPC Monograph 54381.
Blackman, R. L. (1971). Variation in the photoperiodic response within natural populations ofMyzus persicae (Sulz.).Bull. Entomol. Res. 60533.
Burban, C., Fishpool, L. D. C., Fauquet, C., Fargette, D., and Thouvenel, J.-C. (1992). Host-associated biotypes within West African populations of the whiteflyBemisia tabaci (Genn.), (Hom., Aleyrodidae).J. Appl. Ent. 113416.
Byrne, F. J., and Devonshire, A. L. (1991).In vivo inhibition of esterase and acetylcholinesterase activities by profenofos treatments in the tobacco whiteflyBemisia tabaci (Genn.): Implications for routine biochemical monitoring of these enzymes.Pestic. Biochem. Physiol. 40198.
Byrne, F. J., and Devonshire, A. L. (1993). Insensitive acetylcholinesterase and esterase polymorphism in susceptible and resistant populations of the tobacco whiteflyBemisia tabaci (Genn.).Pestic. Biochem. Physiol. 4534.
Byrne, F. J., Cahill, M., Denholm, I., and Devonshire, A. L. (1994). A biochemical and toxicological study of the role of insensitive acetylcholinesterase in organophosphorus resistantBemisia tabaci (Homoptera: Aleyrodidae) from Israel.Bull. Entomol. Res. 42179.
Byrne, F. J., Denholm, I., Birnie, L. C., Devonshire, A. L., and Rowland, M. W. (1992). Analysis of insecticide resistance in the whitefly,Bemisia tabaci (Genn.). In Denholm, I., Devonshire, A. L., and Hollomon, D. (eds.),Achievements and Developments in Combatting Pesticide Resistance Elsevier, London, pp. 165–178.
Campbell, B. C., Duffus, J. E., and Baumann, P. (1993). Determining whitefly species.Science (Technical comments) 2611333.
Costa, H. S., and Brown, J. K. (1991). Variation in biological characteristics and esterase patterns among populations ofBemisia tabaci, and the association of one population with silverleaf symptom induction.Entomol. Exp. Appl. 61211.
Costa, H. S., Brown, J. K., Sivasupramaniam, S., and Bird, J. (1993). Regional distribution, insecticide resistance, and reciprocal crosses between the A and B biotypes ofBemisia tabaci.Insect Sci. Applic. 14255.
Ellman, G. L., Courtney, K. D., Andres, V., and Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity.Biochem. Pharmacol. 788.
Ishaaya, I., Mendelson, Z., Ascher, K. R. S., and Casida, J. E. (1987). Cypermethrin synergism by pyrethroid esterase inhibitors in adults of the whiteflyBemisia tabaci. Pestic. Biochem. Physiol. 28155.
Liu, H. Y., Duffus, J. E., and Cohen, S. (1992).Bemisia tabaci population control by host manipulation and genetics.Proc. 5th Inter. Plant Virus Epidemiol. Symp. (ISPP, Bari, Italy), pp. 227–228.
Loxdale, H. D., and den Hollander, J., eds. (1989). Electrophoretic studies on agricultural pests. Clarendon Press, Oxford.
Perring, T. M., Cooper, A., Kazmer, D. J., Shields, C., and Shields, J. (1991). New strain of sweetpotato whitefly invades California vegetables.Calif. Agric. 4510.
Perring, T. M., Cooper, A. D., Rodriquez, R. J., Farrar, C. A., and Bellows, T. S. (1993). Identification of a whitefly species by genomic and behavioral studies.Science 25974.
Yokomi, R. K., Hoelmer, K. A., and Osborne, L. S. (1990). Relationship between the sweetpotato whitefly and the squash silverleaf disorder.Phytopathology 80895.
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Byrne, F.J., Cahill, M., Denholm, I. et al. Biochemical identification of interbreeding between B-type and non B-type strains of the tobacco whiteflyBemisia tabaci . Biochem Genet 33, 13–23 (1995). https://doi.org/10.1007/BF00554554
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DOI: https://doi.org/10.1007/BF00554554