Abstract
The genes encoding glycolytic enzymes inDrosophila form a group of functionally related genes that may be coordinately regulated and thus controlled by common factors. We have examined the effect of dietary carbohydrates and ethanol on expression of the genes encoding glycerol-3-phosphate dehydrogenase (GPDH), aldolase (ALD), and phosphoglycerate kinase (PGK) inD. melanogaster larvae. GPDH activity and transcript abundance increased in response to ethanol and additional amounts of several different carbohydrates. In addition, the levels of two alternatively processedGpdh transcripts were differentially regulated by the treatments. The nutritional conditions tested had little or no effect on the activities and transcript levels of ALD and PGK. These results indicate that changes in dietary conditions affect expression of specific genes and do not evoke a general response from genes involved in cellular metabolism. The observation that dietary carbohydrates and ethanol increaseGpdh expression without affecting expression ofAld andPgk reinforces previous suggestions that dietary carbon can be diverted by GPDH from glycolytic catabolism into lipid biosynthesis.
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This work was supported by National Institutes of Health Grant GM26830 to D.T.S. and National Institutes of Health Grant AA06702 to B.W.G.
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Lissemore, J.L., Baumgardner, C.A., Geer, B.W. et al. Effect of dietary carbohydrates and ethanol on expression of genes encodingsn-glycerol-3-phosphate dehydrogenase, aldolase, and phosphoglycerate kinase inDrosophila larvae. Biochem Genet 28, 615–630 (1990). https://doi.org/10.1007/BF00553954
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DOI: https://doi.org/10.1007/BF00553954