Abstract
The effects of neuropeptide Y (NPY; 1–36) and NPY fragment (16–36) on nicotinic currents (I ACh) and voltage-dependent calcium currents (I Ca) were studied in bovine chromaffin cells using the whole-cell patchclamp technique. The peak amplitude of inward nicotinic currents was markedly depressed by both NPY(1–36) and NPY (16–36). In contrast, I Ca was unaffected by either NPY(1–36) or NPY(16–36). Both pertussis toxin pretreatment and including GDP[β-S] in the patch pipette solution completely abolished the inhibitory effect of NPY on I ACh It is concluded that inhibition of I ACh probably represents the mechanism by which NPY decreases catecholamine release from adrenal medulla. This effect appears to be mediated by a G-protein-coupled Y2 receptor.
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Nörenberg, W., Illes, P. & Takeda, K. Neuropeptide Y inhibits nicotinic cholinergic currents but not voltage-dependent calcium currents in bovine chromaffin cells. Pflugers Arch. 418, 346–352 (1991). https://doi.org/10.1007/BF00550872
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DOI: https://doi.org/10.1007/BF00550872