Skip to main content
SpringerLink
Log in

Fluorometric assay of histamine in tissues and body fluids: Choice of the purification procedure and identification in the nanogram range

Fluorimetrische Bestimmung von Histamin in Geweben und Körperflüssigkeiten: Wahl eines geeigneten Reinigungsverfahrens und Identifizierung im ng-Bereich

  • Published:
Fresenius' Zeitschrift für analytische Chemie Aims and scope Submit manuscript

Zusammenfassung

Verschiedene Isolierungsverfahren für Histamin zur fluorimetrischen Bestimmung wurden geprüft. Für Histamingehalte von mehr als 1 μg/g Gewebe oder Milliliter Vollblut eignet sich sowohl die Butanolextraktion als auch die Ionenaustausch-Chromatographie an Dowex 50W-X8, für Vollblut des Hundes nur das letztere Verfahren, für Plasma und Magensaft des Menschen nur die Kombination der beiden Isolierungsmethoden. Histamin wurde identifiziert durch Dünnschicht-Chromatographie auf Cellulose, Umsatz durch angereicherte Diaminoxydase und Histaminmethyltransferase, durch die Fluorescenzspektren nach Kondensation mit o-Phthaldialdehyd und durch seine biologische Aktivität. Die Histamingehalte in Geweben und Körperflüssigkeiten des Menschen und einiger Versuchstiere werden mitgeteilt.

Summary

Several procedures for the isolation of histamine from tissues and body fluids were examined. For the fluorometric assay of histamine concentrations higher than 1 μg/g tissue or ml blood the extraction into n-butanol as well as the cation-exchange chromatography on Dowex 50W-X8 proved suitable. The determination of histamine in whole blood of dogs gave valid results only after chromatography on Dowex 50, in human plasma and gastric juice only after the combined application of both purification methods. Histamine was identified by thin-layer chromatography on cellulose, by inactivation in the presence of purified diamine oxidase from pig kidney and purified histamine methyltransferase from guinea-pig brain, by the biological activities and by the fluorescence spectra of the condensation product between histamine and o-phthaldialdehyde. Histamine concentrations in tissues and body fluids of man and some laboratory animals are reported.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Ackermann, D.: Z. Physiol. Chem. 65, 504 (1910).

    Google Scholar 

  2. Barger, G., Dale, H. H.: J. Physiol. (London) 40, 38 (1910).

    Google Scholar 

  3. Barsoum, G. S., Gaddum, J. H.: J. Physiol. (London) 85, 1 (1935).

    Google Scholar 

  4. Brown, D. D., Tomchick, R., Axelrod, J.: J. Biol. Chem. 234, 2948 (1959).

    Google Scholar 

  5. Code, C. F., McIntire, F. C.: Meth. Biochem. Analysis 3, 49 (1956).

    Google Scholar 

  6. Cohn, V. H., Jr., Shore, P. A.: Anal. Biochem. 2, 237 (1961); vgl. diese Z. 189, 377 (1962).

    Google Scholar 

  7. Kremzner, L. T., Pfeiffer, C. C.: Biochem. Pharmacol. 15, 197 (1966).

    Google Scholar 

  8. Lorenz, W., Barth, H., Werle, E.: Arch. Pharmakol. Exp. Pathol. (in press).

  9. —, Doenicke, A., Halbach, S., Krumey, I., Werle, E.: Klin. Wochschr. 47, 154 (1969).

    Google Scholar 

  10. —, Feifel, G., Schmal, A., Hutzel, M., Werle, E.: Klin. Wochschr. 38, 314 (1970).

    Google Scholar 

  11. - Halbach, St.: to be published.

  12. —, Kusche, J., Werle, E.: Z. Physiol. Chem. 348, 561 (1967).

    Google Scholar 

  13. —, Pfleger, K., Werle, E.: Biochem. Pharmacol. 17, 539 (1968).

    Google Scholar 

  14. - Werle, E.: Occurrence, distribution and subcellular localization of histamine in man, animals and plants. In: Intern. Encyclopedia of pharmacology and therapeutics. Oxford: Pergamon Press (in press).

  15. McIntire, F. C.: Handbook exp. pharmacol. (O. Eichler and A. Farah, eds.), vol. 18. Berlin-Heidelberg-New York: Springer 1966.

    Google Scholar 

  16. Mondovi, B., Rotilio, G., Finazzi, A., Scioscia-Santoro, A.: Biochem. J. 91, 408 (1964).

    Google Scholar 

  17. Reuse, J. J.: J. Physiol. (Paris) 40, 289 (1948).

    Google Scholar 

  18. Shelley, W. B., Juhlin, L.: J. Chromatog. 22, 130 (1966); vgl. diese Z. 230, 231 (1967).

    Google Scholar 

  19. Shore, P. A., Burkhalter, A., Cohn, V. H., Jr.: J. Pharmacol. Exp. Therap. 127, 182 (1959); vgl. diese Z. 193, 398 (1963).

    Google Scholar 

  20. Udenfriend, S.: Fluorescence assay in biology and medicine, Vol. II, p. 237. New York: Academic Press 1969.

    Google Scholar 

  21. Werle, E., Lorenz, W.: In Bradykinin and related substances. Cardiovascular, biochemical and neural actions. Plenum Press 1970.

Download references

Author information

Authors and Affiliations

  1. Institute of Clinical Chemistry and Clinical Biochemistry, University of Munich, Germany

    W. Lorenz, L. Benesch, H. Barth, E. Matejka, R. Meyer, J. Kusche, M. Hutzel & E. Werle

Authors
  1. W. Lorenz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Benesch
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Barth
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Matejka
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Meyer
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. Kusche
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. Hutzel
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. E. Werle
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Members of SFB 37 (Restitution und Substitution innerer Organe of Deutsche Forschungsgemeinschaft).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lorenz, W., Benesch, L., Barth, H. et al. Fluorometric assay of histamine in tissues and body fluids: Choice of the purification procedure and identification in the nanogram range. Z. Anal. Chem. 252, 94–98 (1970). https://doi.org/10.1007/BF00546360

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00546360

Keywords

Search

Navigation