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Indication of the metarhodopsin I–II transition by absorption-changes of eriochromblack T

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Abstract

The dye eriochromblack T (erio T), added to an aqueous suspension of bovine retinal outer segments solubilized by digitonin, shows a light-induced absorption-increase at λ=645 nm. Erio T is shown to directly interact with micellar metarhodopsm I and metarhodopsin II. The absorption-changes of erio T can be regarded as an indication of the transition from the metarhodopsin I conformation (with associated Ca2+) to the metarhodopsin II conformation (with associated H+).

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Author notes

  1. Hinderk M. Emrich

    Present address: Max-Planck-Institut für Psychiatrie, Kraepelinstr. 10, D-8000, München 40, Germany

Authors and Affiliations

  1. Max-Volmer-Institut für Physikalische Chemie und Molekularbiologie, Technische UniversitÄt Berlin, Deutschland

    Hinderk M. Emrich & Roland Reich

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  1. Hinderk M. Emrich
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  2. Roland Reich
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Thanks are due to the Deutsche Forschungsgemeinschaft for financial support (Em 18/1-4).

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Emrich, H.M., Reich, R. Indication of the metarhodopsin I–II transition by absorption-changes of eriochromblack T. Biophys. Struct. Mechanism 2, 321–331 (1977). https://doi.org/10.1007/BF00537502

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  • DOI: https://doi.org/10.1007/BF00537502

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