Abstract
Sporocysts collected from the feces of a Palestinian viper (Vipera palaestinae) were administered orally to species of various rodent genera such as Mus, Microtus, Mastomys, Meriones and Oryctolagus. Infections developed only in laboratory mice (Mus musculus). This investigation established the life cycle of Sarcocystis muriviperae in the laboratory. S. muriviperae is described as a new species, based on light and electron microscopic observations and repeated transmission studies. Naturally and experimentally infected Palestinian vipers both excreted structurally identical sporocysts measuring 9.6 Μm (8.8–10.5 Μm) by 12.2 Μm (11.7–12.9 Μm). Sporulation inside the snakes' intestine is completed between 14 and 19 days post-inoculation (p.i.).
Rosette-like schizogonic stages were found in the liver cells of laboratory mice 9–10 days after infection with sporulated sporocysts. Sarcocysts measured up to 1,000 Μm in length on day 36 p.i. and were mainly filled with metrocytes. The septated sarcocysts found 136 or 165 days p.i. reached a length of 5–8 mm and a width of 150–400 Μm. The primary sarcocyst wall formed cauliflower-like branched protrusions about 3.5 Μm in length.
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Abbreviations
- A :
-
Amylopectin granules
- C :
-
conoid
- CM :
-
cyst merozoites
- DB :
-
dense bodies
- DM :
-
developing merozoites
- DO :
-
developing oocyst wall
- GS :
-
ground substance
- HC :
-
host cell
- HO :
-
hollow in the cyst's interior
- L :
-
lipid inclusion
- MC :
-
metrocytes
- MN :
-
micronemes
- MP :
-
micropore
- N :
-
nucleus
- NH :
-
nucleus of the host cell
- OW :
-
oocyst wall
- P :
-
protrusion of PC
- PC :
-
primary cyst wall
- R :
-
rhoptries
- RB :
-
residual body
- RH :
-
residual host cell cytoplasm
- S :
-
schizont
- SP :
-
sporocyst wall
- SU :
-
suture, opening of SP
- TH :
-
thin invagination of PC
- UL :
-
underlying dense material of PC
- UM :
-
unit membrane of PC
References
Beaver PC, Maleckar JR (1981) Sarcocystis singaporensis Zaman and Colley 1976, Sarcocystis villivillosi and Sarcocystis zamani sp.n.: development, morphology and persistence in the laboratory rat, Rattus norvegicus. J Parasitol 67:241–256
Bledsoe B (1979) Sporogony of Sarcocystis idahoensis in the Gopher snake, Pituophis melanoleucus (Daudin). J Parasitol 65:875–879
Bledsoe B (1980) Sarcocystis idahoensis sp.n. in deer mice Peromyscus maniculatus (Wagner) and Gopher snakes Pituophis melanoleucus (Daudin). J Protozool 27:93–102
Brehm H, Frank W (1980) Der Entwicklungskreislauf von Sarcocystis singaporensis Zaman and Colley 1976 im End- und Zwischenwirt. Z Parasitenkd 62:15–30
Frenkel JK, Heydorn AO, Mehlhorn H, Rommel M (1979) Sarcocystinae: Nomina dubia and available names. Z Parasitenkd 58:115–139
Häfner U, Matuschka FR (1984) Life cycle studies on Sarcocystis dirumpens sp.n. with regard to host specificity. Z Parasitenkd 70:715–720
Heydorn AO, Gestrich R, Mehlhorn H, Rommel M (1975) Proposal for a new nomenclature of the Sarcosporidia. Z Parasitenkd 48:73–82
Lavier G (1941) Description d'Isospora fragilis L. Leger 1904, coccidie intestinale de Vipera aspis. Bull Soc Path Exot Filiales 34:25–30
Léger L (1904) Protozoaires parasites des vipères. B Mens Ass France Avang 9:208
Matuschka FR (1987) Reptiles as intermediate and/or final hosts of Sarcosporidia. Parasitol Res 73:22–32
Mehlhorn H, Heydorn AO (1978) The sarcosporidia (Protozoa, Sporozoa) life cycle and fine structure. Adv Parasitol 16:43–92
Mehlhorn H, Matuschka FR (1986) Ultrastructural studies of the development of Sarcocystis clethrionomyelaphis within its final and intermediate hosts. Protistologica 22:97–104
Mendelssohn H (1963) On the biology of the venomous snakes of Israel. Isr J Zool 12:143–170
Mendelssohn H, Golani I, Marder U (1971) Agricultural development and the distribution of venomous snakes and snake bite in Israel. In: de Vries A, Koch E (eds), Toxins of Animal and Plant origin, Gordon, Breach Inc, London, pp 3–15
Munday BL, Mason HW (1980) Sarcocystis and related organisms in Australian wildlife: III. Sarcocystis murinotechis sp.n. Life cycle in rats (Rattus, Pseudomys, and Mastocomys spp.) and tiger snakes (Notechis ater). J Wildl Dis 16:83–87
Ovezmukhammedov A (1968) Ein neues Coccid Isospora ashkhabadensis sp.n. bei Vipera lebentina L. Parazitologija 2:375–376 (in Russian)
Ruiz A, Frenkel IK (1976) Recognition of cyclic transmission of Sarcocystis muris by cats. J Infect Dis 133:409–418
Rzepczyk C (1974) Evidence of a rat snake cycle for Sarcocystis. Int J Parasitol 4:447–449
Schnieder T, Trautwein G, Rommel M (1984) Untersuchungen zur Persistenz der Zysten von Sarcocystis miescheriana in der Muskulatur des Schweines nach ein- und mehrmaliger Infektion. Berl Münch Tierärztl Wochenschr 97:356–359
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The study was supported by grants from the “Deutsche Forschungsgemeinschaft”, the “Ständige Kommission für Forschung und wissenschaftlichen Nachwuchs der Freien Universität Berlin” and of the Egyptian government
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Matuschka, F.R., Heydorn, A.O., Mehlhorn, H. et al. Experimental transmission of Sarcocystis muriviperae n. sp. to laboratory mice by sporocysts from the Palestinian viper (Vipera palaestinae): A light and electron microscope study. Parasitol Res 73, 33–40 (1987). https://doi.org/10.1007/BF00536333
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DOI: https://doi.org/10.1007/BF00536333