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Use of water-insoluble enzyme derivatives in biochemical analysis and separation

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Summary

A brief review of the most commonly employed preparative procedures for water-insoluble enzyme derivatives is given, followed by a description of the various factors which can affect the behaviour and the apparent kinetic parameters of an immobilized enzyme system. Current developments in the application of water-insoluble enzyme derivatives in biochemical analysis and separation are dealt with.

Zusammenfassung

Ein Überblick über die gebräuchlichsten Methoden zur Herstellung wasserunslöslicher Enzymderivate wird gegeben. Anschließend werden die verschiedenen Faktoren beschrieben, die das Verhalten und die scheinbaren kinetischen Parameter eines immobilisierten Enzymsystems beeinflussen. Die Anwendungsmöglichkeiten wasserunlöslicher Enzymderivate in der biochemischen Analytik werden besprochen.

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  1. Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel

    Leon Goldstein & Ephraim Katchalski

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  1. Leon Goldstein
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  2. Ephraim Katchalski
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Goldstein, L., Katchalski, E. Use of water-insoluble enzyme derivatives in biochemical analysis and separation. Z. Anal. Chem. 243, 375–396 (1968). https://doi.org/10.1007/BF00530712

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  • DOI: https://doi.org/10.1007/BF00530712

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