Summary
The enzyme aldolase is isolated and crystallized from human breast tumor. The purification of the enzyme involved methanol fractionation, exposure to a temperature of 38°C, adsorption and elution from aluminium hydroxide gel, and precipitation followed by crystallization from ammonium sulfate.
Zusammenfassung
Aldolase wurde aus einem Mammacarcinom isoliert und kristallisiert. Die angewendete Methode besteht in Fraktionierung in Methanol, Einwirkung einer Temperatur von 38°, Adsorption und Elution aus einem Aluminiumhydroxyd-Gel und Präcipitation gefolgt von Kristallisation aus Ammoniumsulfat.
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Hakim, A.A. Breast tumor aldolase. Z Krebs-forsch 62, 647–652 (1959). https://doi.org/10.1007/BF00528215
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DOI: https://doi.org/10.1007/BF00528215