Summary
Several cDNA clones encoding the complete subunit II and III precursor polypeptides of the photosystem I reaction center were isolated from two spinach lambda gtll expression libraries by immunoscreening and homologous hybridization. The identity of the recombinant cDNAs was verified by an N-terminal amino acid sequence of 14 and 20 residues for the respective mature spinach proteins. The ca. 880 nucleotide long sequence and derived amino acid sequence for subunit II predict a precursor of 23.2 kDa (212 residues) and a positively charged, mature protein of 17.9 kDa (162 residues). The corresponding data for subunit III are ca. 710 nucleotides (cDNA), 13.4 kDa (125 residues, precursor polypeptide) and, again, a positively charged, mature protein of 9.7 kDa (91 residues). Secondary structure predictions indicate that both subunits are extramembraneous components of photosystem I. Subunit II is probably located on the matrix-side, subunit III in the lumen of stroma lamellae which is consistent both with biochemical findings and the proposed roles of these proteins in the electron transition from and to photosystem I, respectively. Major transcripts of 1.1 kb (subunit II) and 0.8 kb (subunit III) have been observed by RNA-DNA hybridization.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bengis C, Nelson N (1975) J Biol Chem 250:2783–2788
Bengis C, Nelson N (1977) J Biol Chem 252:4564–4569
Birnboim HC, Doly J (1979) Nucleic Acids Res 7:1513–1534
Chua N-H, Schmidt GW (1979) J Cell Biol 81:461–483
Dean C, Tamaki S, Dunsmuir P, Favreau M, Katayama C, Dooner H, Bedbrook J (1986) Nucleic Acids Res 14:2229–2240
Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13
Fish LE, Kück U, Bogorad L (1985) J Biol Chem 260:1413–1421
Golbeck JH, Parrett KG, McDermott AE (1987) Biochim Biophys Acta 893:149–169
Haehnel N, Pröper A, Krause J (1980) Biochim Biophys Acta 593:384- 399
Haehnel N (1984) Annu Rev Plant Physiol 35:659–693
Haworth P, Watson JL, Arntzen CJ (1983) Biochim Biophys Acta 724:151–158
Hayashida N, Matsubayashi T, Shinozaki K, Sugiura M, Inoue K, Hiyama T (1987) Curr Genet 12:247–250
Heijne G van (1986) Nucleic Acids Res 14:4683–4690
Heijne G van, Steppuhn J, Herrmann RG (1988) Eur J Biochem, (in press)
Herrmann RG, Westhoff P, Alt J, Tittgen J, Nelson N (1985) In: Vloten-Doting L van, Groot GSP, Hall TC (eds) Molecular form and function of the plant genome. Plenum Press, New York, pp 233–256
Hoffman NE, Pichersky E, Maliv VS, Ko K, Cashmore AR (1988) Plant Mol Biol 10:435–445
Høj PB, Møller BL (1986) J Biol Chem 261:14292–14300
Høj PB, Svendsen J, Scheller HV, Møller BL (1987) J Biol Chem 262:12676–12684
Huyhn TV, Young RA, Davis RW (1985) In: Glover DM (ed) DNA cloning: a practical approach, vol I. IRL-Press, Oxford Washington DC, pp 48–88
Jansen T, Rother C, Steppuhn J, Reinke H, Beyreuther K, Jansson C, Anderson B, Herrmann RG (1987) FEBS Lett 216:234–240
Kirsch W, Seyer P, Herrmann RG (1986) Curr Genet 10:843–855
Kozak M (1987) J Mol Biol 196:947–950
Kyte J, Doolittle RF (1982) J Biol 157:105–132
Lagoutte B (1988) FEBS Lett 232:275–280
Lam E, Ortiz W, Mayfield S, Malkin R (1984) Plant Physiol 74:650–655
Lautner A, Klein R, Ljungberg U, Reiländer H, Bartling D, Andersson B, Reinke H, Beyreuther K, Herrmann RG (1988) J Biol Chem 263:10077–10081
Lehmbeck J, Rasmussen OF, Bookjans GB, Jepsen BR, Stummann BM, Henningsen KW (1986) Plant Mol Biol 7:3–10
Malkin R (1987) In: Barber J (ed) The light reactions. Elsevier,Amsterdam New York, pp 495–526
Merati G, Zanettti G (1987) FEBS Lett 215:37–40
Mullet JE, Burke JJ, Arntzen CJ (1980) Plant Physiol 65:814–822
Nechushtai R, Nelson N (1981) J Biol Chem 256:11624–11628
Nechushtai R, Nelson N, Mattoo AK, Edelman M (1981) FEBS Lett 125:115–119
Nechushtai R, Nourizadeh SD, Thornber JP (1986) Biochim Biophys Acta 848:193–200
Nelson N, Notsani BE (1977) In: Packer L, Papageorgiou GC, Trebst A (eds) Bioenergetics of membranes. Elsevier, Amsterdam New York, pp 233–244
Oh-oka H, Takahashi Y, Wada K, Matsubara H, Ohyama K, Ozeki H (1987) FEBS Lett 218:52–54
Ortiz W Lam E, Ghirardi M, Malkin R (1984) Biochim Biophys Acta 776:505–509
Proudfoot N (1982) Nature 298:516–517
Reed KC, Mann DA (1985) Nucleic Acids Res 13:7207–7221
Robinson C, Ellis RJ (1984) Eur J Biochem 142:337–342
Rother C, Jansen T, Tyagi A, Tittgen J, Herrmann RG (1986) Curr Genet 11:171–176
Sakurai H, San Pietro A (1985) J Biochem 98:69–76
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Schmidt GW, Mishkind ML (1986) Annu Rev Biochem 55:879–912
Smeekens S, de Groot M, van Binsbergen J, Weisbeek P (1985) Nature 317:456–458
Steinmüller K, Apel K (1986) Plant Mol Biol 7:87–94
Steppuhn J, Hermans J, Nechushtai R, Ljungberg U, Thümmler F, Lottspeich F, Herrmann RG (1988) FEBS Lett 237:218–224
Tittgen J, Hermans J, Steppuhn J, Jansen T, Jansson C, Andersson B, Nechushtai R, Nelson N, Herrmann RG (1986) Mol Gen Genet 204:258–265
Tyagi A, Hermans J, Steppuhn J, Jansson C, Vater F, Herrmann RG (1987) Mol Gen Genet 207:288–293
Vallejos RH, Chan RL, Ceccarelli EA, Serrano A, Soncini FC (1987) In: Biggins J (ed) Progress in photosynthesis research, vol III. Nijhoff, Dordrecht, pp 439–442
Vierling E, Alberte RS (1983) Plant Physiol 72:625–633
Westhoff P, Alt J, Nelson N, Bottomley W, Bünemann H, Herrmann RG (1983) Plant Mol Biol 2:95–107
Wynn RM, Malkin R (1988) Biochemistry 27:5863–5869
Zanetti G, Merati G (1987) Eur J Biochem 169:143–146
Zanetti G, Morelli D, Ronchi S, Negri A, Aliverti A, Curti B (1988) Biochemistry 27:3753–3759
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Münch, S., Ljungberg, U., Steppuhn, J. et al. Nucleotide sequences of eDNAs encoding the entire precursor polypeptides for subunits II and III of the photosystem I reaction center from spinach. Curr Genet 14, 511–518 (1988). https://doi.org/10.1007/BF00521277
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00521277