Abstract
Two types of α-d-mannosidase isozymes are present in human white blood cells, human diploid fibroblasts, and HeLa cells. One of these (the S isozyme) constitutes the major α-d-mannosidase of the human cells, has a pH optimum of 4.4, and is associated with lysosomes. The other (the F isozyme) is most active at pH 6, is acid labile, and is located in the soluble portion of the cytoplasm. The expression of human lysosomal α-d-mannosidase was examined in man-hamster hybrid clones, and was found to be concordant with that of phosphohexose isomerase in 54 of 55 primary clones. A locus specifying human lysosomal α-d-mannosidase has therefore been assigned to chromosome 19.
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This work was supported by NIH Grants HD 04807-07 and HD 06285-04 and by a research grant (5 PO 1 HB 06276-04) to the Mental Retardation Research Center of the Children's Hospital Medical Center, Boston, Massachusetts, from the NIH.
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Ingram, P.H., Bruns, G.A.P., Regina, V.M. et al. Expression of α-d-mannosidase in man-hamster somatic cell hybrids. Biochem Genet 15, 455–476 (1977). https://doi.org/10.1007/BF00520191
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DOI: https://doi.org/10.1007/BF00520191