Summary
The molecular weight of the putative calcium channel in guinea-pig brain membranes, labelled with [3H]-nimodipine, has been determined in situ by target size analysis. Irradiation of guinea-pig brain membranes at 153–173 K with 10 MeV electrons reduced the density of [3H]-nimodipine binding sites without affecting the equilibrium dissociation constant. The decay of the calcium channel blocker binding site density as a function of radiation dose fits to a monoexponential function. Application of the target size theory gives a molecular weight of 185,000. [3H]-Flunitrazepam labelled benzodiazepine receptor target size was measured as an internal control. The molecular weight of the benzodiazepine receptors was 76,000, which is in good agreement with published results.
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Ferry, D.R., Goll, A. & Glossmann, H. Putative calcium channel molecular weight determination by target size analysis. Naunyn-Schmiedeberg's Arch. Pharmacol. 323, 292–297 (1983). https://doi.org/10.1007/BF00512466
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DOI: https://doi.org/10.1007/BF00512466