Summary
For the efficient production of l-alanine from ammonium fumarate using the aspartase activity of immobilized Escherichia coli cells and l-aspartate β-decarboxylase activity of immobilized Pseudomonas dacunhae cells, alanine racemase and fumarase activities should be eliminated. We investigated various procedures to eliminate these side reactions, and found that both activities of intact E. coli cells could be eliminated by treating the culture broth at pH 5.0 and 45° C for 1 h, and those of intact P. dacunhae cells could be eliminated by treating the culture broth at pH 4.75 and 30° C for 1 h. Further, it was confirmed that l-alanine was efficiently produced using these two immobilized pH-treated microorganisms.
Similar content being viewed by others
References
Bock RM, Alberty RA (1953) Studies of the enzyme fumarase. I. Kinetics and equilibrium. J Am Chem Soc 75:1921–1925
Brumby PE, Massey V (1968) d-Amino acid oxidase from pig kidney. Biochem Prep 12:29–41
Chibata I, Kakimoto T, Kato J (1965) Enzymatic production of l-alanine by Pseudomonas dacunhae. J. Appl Microbiol 13:638–645
Chibata I, Tosa T, Sato T (1974) Immobilized aspartase-containing microbial cells: preparation and enzymatic properties. Appl Microbiol 27:878–885
Friedmann TE, Haugen GE (1943) Pyruvic acid. II. The determination of keto acids in blood and urine. J Biol Chem 147:415–442
Goodman AE, Stark JB (1957) Rapid method for determination of malic acid. Anal Chem 29:283–287
Henderson LM, Snell EE (1948) A uniform medium for determination of amino acids with various microorganisms. J Biol Chem 172:15–29
Nishida Y, Sato T, Tosa T, Chibata I (1979) Immobilization of Escherichia coli cells having aspartase activity with carrageenan and locust bean gum. Enzyme Microb Technol 1:95–99
Sato T, Mori T, Tosa T, Chibata I, Furui M, Yamashita K, Sumi A (1975) Engineering analysis of continuous production of l-aspartic acid by immobilized Escherichia coli cells in fixed beds. Biotechnol Bioeng 17:1797–1804
Snell EE (1957) Microbiological determination of amino acids. In: Colowick SP, Kaplan NO (eds) Methods in Enzymology, vol 3. Academic, New York, p 477–492
Takamatsu S, Yamamoto K, Tosa T, Chibata I (1981) Stabilization of l-aspartate β-decarboxylase activity of Pseudomonas dacunhae immobilized with carrageenan. J Ferment Technol 59:489–493
Takata I, Yamamoto K, Tosa T, Chibata I (1980) Immobilization of Brevibacterium flavum with carrageenan and its application for continuous production of l-malic acid. Enzyme Microb Technol 2:30–36
Tosa T, Sato T, Mori T, Chibata I (1974) Basic studies for continuous production of l-aspartic acid by immobilized Escherichia coli cells. Appl Microbiol 27:886–889
Yamamoto K, Sato T, Tosa T, Chibata I (1974) Continuous production of urocanic acid by immobilized Achromobacter liquidum cells. Biotechnol Bioeng 16:1601–1610
Yamamoto K, Tosa T, Chibata I (1980) Continuous production of l-alanine using Pseudomonas dacunhae immobilized with carrageenan. Biotechnol Bioeng 22:2045–2054
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Takamatsu, S., Umemura, I., Yamamoto, K. et al. Production of l-alanine from ammonium fumarate using two immobilized microorganisms. European J. Appl. Microbiol. Biotechnol. 15, 147–152 (1982). https://doi.org/10.1007/BF00511238
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00511238