Summary
An amylolytic obligate thermophile producing high amounts of extracellular pullulanase was isolated from soil. The isolate (KP 1064) that grew from 45°C to 69°C was identified as a strain of Bacillus stearothermophilus. Maximal enzyme activity was achieved after 18 h of shaking cultivation at 60°C and at an initial pH of 7.1–7.5, on a medium composed of 1.2% (w/v) soluble starch, 1.4%–1.6% peptone, 0.3% yeast extract, 0.3% K2HPO4, and 0.1% KH2PO4. Pullulanase synthesis was significantly enhanced by dextrin, amylopectin, soluble starch as well as maltose, but not at all by pullulan. A large enzyme accumulation in the culture broth took place in the early stationary phase of growth, but the exoenzyme activity rapidly disappeared in the late stage of this period with a rate of 0.074 unit decreased/ml of culture/10h.
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Suzuki, Y., Chishiro, M. Production of extracellular thermostable pullulanase by an amylolytic obligately thermophilic soil bacterium, Bacillus stearothermophilus KP 1064. European J. Appl. Microbiol. Biotechnol. 17, 24–29 (1983). https://doi.org/10.1007/BF00510567
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DOI: https://doi.org/10.1007/BF00510567