Summary
Cerebral energy metabolism was studied in the isolated perfused rat brain after 6-aminonicotinamide (6-AN; 35 mg/kg i.p.) administered to the intact animals 7 hrs before perfusion was started. The metabolic alterations in the isolated rat brains were such as reported for rat and mouse brain in vivo: Inhibition of 6-phosphogluconate dehydrogenase was followed by an accumulation of 6-phosphogluconate, leading to a decreased activity of glucosephosphate isomerase. This was reflected by increased levels of glucose and glucose 6-phosphate and decreased levels of fructose 6-phosphate, pyruvate and lactate. Since the concentration of lactate in the perfusate of the isolated brain was also lowered, 6-AN must have reduced the glycolytic flux rate.
This is a preview of subscription content, log in via an institution to check access.
References
Andjus, R., Suhara, K., Sloviter, H. A.: An isolated perfused rat brain preparation, its spontaneous and stimulated activity. J. appl. Physiol. 22, 1033–1039 (1967)
Bergmeyer, H.U.: Methoden der enzymatischen Analyse, 2. Aufl. Weinheim: Verlag Chemie 1970
Fleck, W., Krieglstein, J., Urban, W.: Zwei Apparaturen zur Perfusion des isolierten Rattenhirns. Arzneimittel-Forsch. 22, 1225–1230 (1972)
Grüner, J., Krieglstein, J., Rieger, H.: Comparison of the effects of chloral hydrate and trichloroethanol on the EEG of the isolated perfused rat brain. Naunyn-Schmiedeberg's Arch. Pharmacol. 277, 333–348 (1973)
Herken, H., Keller, K., Kolbe, H., Lange, K., Schneider, H.: Experimentelle Myelopathie—Biochemische Grundlagen ihrer cellulären Pathogenese. Klin. Wschr. 51, 644–657 (1973)
Herken, H., Lange, K.: Blocking of pentose phosphate pathway in the brain of rats by 6-aminonicotinamide. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 263, 496–499 (1969)
Herken, H., Lange, K., Kolbe, H.: Brain disorders induced by pharmacological blockade of the pentose phosphate pathway. Biochem. biophys. Res. Commun. 36, 93–100 (1969)
Kauffman, F. C., Johnson, E. C.: Cerebral energy reserves and glycolysis in neural tissue of 6-aminonicotinamide-treated mice. J. Neurobiol. 5, 379–392 (1974)
Keller, K., Kolbe, H., Lange, K., Herken, H.: Behaviour of the glycolytic system of rat brain and kidney in vivo after inhibition of the glucose-phosphate isomerase. II. Substrate concentrations under the influence of ischemia, 6-aminonicotinamide, and 2-deoxyglucose. Hoppe-Seyler's Z. physiol. Chem. 353, 1389–1400 (1972)
Krieglstein, G., Krieglstein, J., Stock, R.: Suitability of the isolated perfused rat brain for studying effects on cerebral metabolism. Naunyn-Schmiedeberg's Arch. Pharmacol. 275, 124–134 (1972)
Lange, K., Kolbe, H., Keller, K., Herken, H.: Der Kohlenhydratstoffwechsel des Gehirns nach Blockade des Pentose-Phosphat-Weges durch 6-Aminonicotinsäureamid. Hoppe-Seyler's Z. physiol. Chem. 351, 1241–1252 (1970)
Sternberg, I.S., Philips, F.S.: 6-Aminonicotinamide and acute degenerative changes in the central nervous system. Science 127, 644–646 (1958)
Wolf, A., Cowen, D., Geller, L. M.: The effects of an antimetabolite, 6-aminonicotinamide, on the central nervous system. Trans. Amer. Neurol. Ass. 84, 140–145 (1959)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Krieglstein, J., Stock, R. Decreased glycolytic flux rate in the isolated perfused rat brain after pretreatment with 6-aminonicotinamide. Naunyn-Schmiedeberg's Arch. Pharmacol. 290, 323–327 (1975). https://doi.org/10.1007/BF00510561
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00510561